BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18998

Title: Allatide O4 conformation 2

Deposition date: 2013-02-03 Original release date: 2015-01-12

Authors: BAI, YANG

Citation: BAI, YANG; PERVUSHIN, KONSTANTIN. "Allatides: Smallest Proteinaceous -Amylase Inhibitors from Allamanda oenotheraefolia and Their Expression in Apocynaceae Plants"  J. Biol. Chem. ., .-..

Assembly members:
allatide_conf2, polymer, 30 residues, 3299.954 Da.

Natural source:   Common Name: Eudicots   Taxonomy ID: 52817   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Allamanda oenotheraefolia

Experimental source:   Production method: purified from the natural source   Host organism: Allamanda oenotheraefolia

Entity Sequences (FASTA):
allatide_conf2: CIAHYGKCDGIINQCCDPWL CTPPIIGFCL

Data sets:
Data typeCount
1H chemical shifts182

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Allatide O4 conformation 21

Entities:

Entity 1, Allatide O4 conformation 2 30 residues - 3299.954 Da.

1   CYSILEALAHISTYRGLYLYSCYSASPGLY
2   ILEILEASNGLNCYSCYSASPPROTRPLEU
3   CYSTHRPROPROILEILEGLYPHECYSLEU

Samples:

sample_1: allatide_conf20.5 – 1.0 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K; temperature controller setting: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.0, Keller and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 18999
PDB
GB AGN03149 AGN03153