BMRB Entry 19043
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PDB ID: 2m5a
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19043
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Title: Protein A binding by an engineered Affibody molecule PubMed: 23924760
Deposition date: 2013-02-19 Original release date: 2013-08-14
Authors: Hard, Torleif
Citation: Lindborg, Malin; Dubnovitsky, Anatoly; Olesen, Kenneth; Bjorkman, Tomas; Abrahmsen, Lars; Feldwisch, Joachim; Hard, Torleif. "High-affinity binding to staphylococcal protein A by an engineered dimeric Affibody molecule." Protein Eng. Des. Sel. 26, 635-644 (2013).
Assembly members:
Z_domain, polymer, 58 residues, 6648.390 Da.
ZpA963, polymer, 58 residues, 6394.143 Da.
Natural source: Common Name: Staphylococcus aureus Taxonomy ID: 1280 Superkingdom: Bacteria Kingdom: not available Genus/species: Staphylococcus aureus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Z_domain: VDNKFNKEQQNAFYEILHLP
NLNEEQRNAFIQSLKDDPSQ
SANLLAEAKKLNDAQAPK
ZpA963: VDNKFNKETQEASWEIFTLP
NLNGRQVAAFISSLLDDPSQ
SANLLAEAKKLNDAQAPK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 507 |
| 15N chemical shifts | 135 |
| 1H chemical shifts | 835 |
Additional metadata:
Related Database Links:
| BMRB | 4023 |
| PDB | 1H0T 1LP1 1Q2N 2M5A 2SPZ 4GWP 4GWQ |
| DBJ | BAC76617 BAC76619 BAL27690 BAL27694 BAL27697 |
| EMBL | CAA49867 CAA65431 |
| GB | AAA72944 AAA73085 AAB00807 AAR10383 AAR10384 |
| REF | WP_052996913 |
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