BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19077

Title: 1H, 13C and 15N backbone and side-chain resonance assignments of the N-terminal ubiquitin-binding domain of the human deubiquitinase USP28   PubMed: 23754699

Deposition date: 2013-03-06 Original release date: 2014-02-13

Authors: Wen, Yi; Cui, Rong; Zhang, Huaqun; Zhang, Naixia

Citation: Wen, Yi; Cui, Rong; Zhang, Huaqun; Zhang, Naixia. "(1)H, (13)C and (15)N backbone and side-chain resonance assignments of the N-terminal ubiquitin-binding domains of the human deubiquitinase Usp28."  Biomol. NMR Assignments ., .-. (2013).

Assembly members:
USP28, polymer, 137 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
USP28: GSMTAELQQDDAAGAADGHG SSCQMLLNQLREITGIQDPS FLHEALKASNGDITQAVSLL TDERVKEPSQDTVATEPSEV EGSAANKEVLAKVIDLTHDN KDDLQAAIALSLLESPKIQA DGVDKLAAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts487
15N chemical shifts111
1H chemical shifts764

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1USP28 monomer1

Entities:

Entity 1, USP28 monomer 137 residues - Formula weight is not available

The first two residues GS and the last fifteen residues VDKLAAALEHHHHHH are cloning artifacts.

1   GLYSERMETTHRALAGLULEUGLNGLNASP
2   ASPALAALAGLYALAALAASPGLYHISGLY
3   SERSERCYSGLNMETLEULEUASNGLNLEU
4   ARGGLUILETHRGLYILEGLNASPPROSER
5   PHELEUHISGLUALALEULYSALASERASN
6   GLYASPILETHRGLNALAVALSERLEULEU
7   THRASPGLUARGVALLYSGLUPROSERGLN
8   ASPTHRVALALATHRGLUPROSERGLUVAL
9   GLUGLYSERALAALAASNLYSGLUVALLEU
10   ALALYSVALILEASPLEUTHRHISASPASN
11   LYSASPASPLEUGLNALAALAILEALALEU
12   SERLEULEUGLUSERPROLYSILEGLNALA
13   ASPGLYVALASPLYSLEUALAALAALALEU
14   GLUHISHISHISHISHISHIS

Samples:

sample_1: D20 10%; H20 90%; USP-28, [U-15N], 0.8 – 1.0 mM; Na2HPO4/NaH2PO4 20 mM; NaCl 100 mM; DTT 2 mM; NaNH3 0.02%

sample_2: D20 10%; H20 90%; USP-28, [U-15N; U-13C], 0.8 – 1.0 mM; Na2HPO4/NaH2PO4 20 mM; NaCl 100 mM; DTT 2 mM; NaNH3 0.02%

sample_3: D20 100%; USP-28, [U-15N; U-13C], 0.8 – 1.0 mM; Na2HPO4/NaH2PO4 20 mM; NaCl 100 mM; DTT 2 mM; NaNH3 0.02%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 18560
PDB
DBJ BAG65214
GB AAH65928 AAK58565 ACA06098 ACA06099 AIC60511
REF NP_065937 XP_001085291 XP_002799842 XP_003253212 XP_004052203
SP Q96RU2

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts