BMRB Entry 19115
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19115
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Title: Full-length mercury transporter protein MerF in lipid bilayer membranes PubMed: 23763519
Deposition date: 2013-03-27 Original release date: 2013-07-01
Authors: Lu, George; Tian, Ye; Vora, Nemil; Marassi, Francesca; Opella, Stanley
Citation: Lu, George; Tian, Ye; Vora, Nemil; Marassi, Francesca; Opella, Stanley. "The Structure of the Mercury Transporter MerF in Phospholipid Bilayers: A Large Conformational Rearrangement Results from N-Terminal Truncation" J. Am. Chem. Soc. 135, 9299-9302 (2013).
Assembly members:
MerF, polymer, 81 residues, 8670.492 Da.
Natural source: Common Name: Enterobacteria Taxonomy ID: 582 Superkingdom: Bacteria Kingdom: not available Genus/species: Morganella morganii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MerF: MKDPKTLLRVSIIGTTLVAL
SSFTPVLVILLGVVGLSALT
GYLDYVLLPALAIFIGLTIY
AIQRKRQADASSTPKFNGVK
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 116 |
| 15N chemical shifts | 58 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MerF | 1 |
Entities:
Entity 1, MerF 81 residues - 8670.492 Da.
Residue 81 Z is homoserine resulting from cyanogen bromide cleavage
| 1 | MET | LYS | ASP | PRO | LYS | THR | LEU | LEU | ARG | VAL | ||||
| 2 | SER | ILE | ILE | GLY | THR | THR | LEU | VAL | ALA | LEU | ||||
| 3 | SER | SER | PHE | THR | PRO | VAL | LEU | VAL | ILE | LEU | ||||
| 4 | LEU | GLY | VAL | VAL | GLY | LEU | SER | ALA | LEU | THR | ||||
| 5 | GLY | TYR | LEU | ASP | TYR | VAL | LEU | LEU | PRO | ALA | ||||
| 6 | LEU | ALA | ILE | PHE | ILE | GLY | LEU | THR | ILE | TYR | ||||
| 7 | ALA | ILE | GLN | ARG | LYS | ARG | GLN | ALA | ASP | ALA | ||||
| 8 | SER | SER | THR | PRO | LYS | PHE | ASN | GLY | VAL | LYS | ||||
| 9 | LYS |
Samples:
sample_1: MerF, [U-99% 13C; U-99% 15N], 2.0 – 3.0 mg; 1,2-di-O-tetradecyl-sn-glycero-3-phosphocholine10.0 – 15.0 mg; MES 20 mM; H2O 100%
sample_conditions_1: pH: 6.0; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DARR | sample_1 | solid | sample_conditions_1 |
| 2D NC correlation | sample_1 | solid | sample_conditions_1 |
| 3D HnNCa SLF | sample_1 | solid | sample_conditions_1 |
| 3D HnNCo SLF | sample_1 | solid | sample_conditions_1 |
| 3D HcCxCx SLF | sample_1 | solid | sample_conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 700 MHz
- Varian DD2 800 MHz