BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19195

Title: alfa-actinin from parasite Entamoeba histolytica

Deposition date: 2013-04-26 Original release date: 2013-06-04

Authors: Persson, Cecilia; Mayzel, Maxim; Karlsson, Goran; Backman, Lars

Citation: Persson, Cecilia; Mayzel, Maxim; Karlsson, Goran; Backman, Lars. "alfa-actinin from parasite Entamoeba histolytica"  Not known ., .-..

Assembly members:
entity, polymer, 144 residues, 15924.354 Da.

Natural source:   Common Name: Entamoeba Histolytica   Taxonomy ID: 5759   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Entamoeba Histolytica

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: GSSSGVTAEQMQEFKQSFDA FDGNHDGILDKLEFRSCLSS MGLIDIDFTGGEDAQYDAIY NNVTKGENGVSFDNYVQYMK EKNDENPSPEQLNEIFSTIA AGKDSITETDMQKAGMSAEQ IEYVKANLPQKGDGYDYAAW VKTN

Data sets:
Data typeCount
13C chemical shifts537
15N chemical shifts136
1H chemical shifts869

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alfa-actinin from parasite Entamoeba histolytica1

Entities:

Entity 1, alfa-actinin from parasite Entamoeba histolytica 144 residues - 15924.354 Da.

1   GLYSERSERSERGLYVALTHRALAGLUGLN
2   METGLNGLUPHELYSGLNSERPHEASPALA
3   PHEASPGLYASNHISASPGLYILELEUASP
4   LYSLEUGLUPHEARGSERCYSLEUSERSER
5   METGLYLEUILEASPILEASPPHETHRGLY
6   GLYGLUASPALAGLNTYRASPALAILETYR
7   ASNASNVALTHRLYSGLYGLUASNGLYVAL
8   SERPHEASPASNTYRVALGLNTYRMETLYS
9   GLULYSASNASPGLUASNPROSERPROGLU
10   GLNLEUASNGLUILEPHESERTHRILEALA
11   ALAGLYLYSASPSERILETHRGLUTHRASP
12   METGLNLYSALAGLYMETSERALAGLUGLN
13   ILEGLUTYRVALLYSALAASNLEUPROGLN
14   LYSGLYASPGLYTYRASPTYRALAALATRP
15   VALLYSTHRASN

Samples:

sample_1: The protein, [U-100% 13C; U-100% 15N], 1.5 mM; sodium phosphate 25 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

ANALYSIS v2.2, CCPN - assignment and data analysis

DANGLE v1.1 -

CYANA v2.2, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

PDB
GB EAL47897 EKE40069 EMD43687 EMH76125 EMS12580
REF XP_008857596 XP_653283

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts