BMRB Entry 19201
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19201
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Title: TAX1BP1 UBZ1+2 PubMed: 24239949
Deposition date: 2013-04-29 Original release date: 2013-12-02
Authors: Ceregido, M. Angeles; Spinola, Mercedes; Buts, Lieven; Bravo, Jeronimo; van Nuland, Nico
Citation: Ceregido, M. Angeles; Spinola Amilibia, Mercedes; Buts, Lieven; Rivera-Torres, Jose; Garcia-Pino, Abel; Bravo, Jeronimo; van Nuland, Nico. "The Structure of TAX1BP1 UBZ1+2 Provides Insight into Target Specificity and Adaptability." J. Mol. Biol. 426, 674-690 (2014).
Assembly members:
TAX1BP1_UBZ1+2, polymer, 69 residues, 8332.372 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TAX1BP1_UBZ1+2: GPHMDVHKKCPLCELMFPPN
YDQSKFEEHVESHWKVCPMC
SEQFPPDYDQQVFERHVQTH
FDQNVLNFD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 295 |
| 15N chemical shifts | 72 |
| 1H chemical shifts | 466 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TAX1BP1_UBZ1+2 | 1 |
| 2 | ZINC ION_1 | 2 |
| 3 | ZINC ION_2 | 2 |
Entities:
Entity 1, TAX1BP1_UBZ1+2 69 residues - 8332.372 Da.
| 1 | GLY | PRO | HIS | MET | ASP | VAL | HIS | LYS | LYS | CYS | ||||
| 2 | PRO | LEU | CYS | GLU | LEU | MET | PHE | PRO | PRO | ASN | ||||
| 3 | TYR | ASP | GLN | SER | LYS | PHE | GLU | GLU | HIS | VAL | ||||
| 4 | GLU | SER | HIS | TRP | LYS | VAL | CYS | PRO | MET | CYS | ||||
| 5 | SER | GLU | GLN | PHE | PRO | PRO | ASP | TYR | ASP | GLN | ||||
| 6 | GLN | VAL | PHE | GLU | ARG | HIS | VAL | GLN | THR | HIS | ||||
| 7 | PHE | ASP | GLN | ASN | VAL | LEU | ASN | PHE | ASP |
Entity 2, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: UBZ1+2, [U-98% 13C; U-98% 15N], 0.5 – 1.0 mM; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 0 M; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| (HB)CB(CGCD)HD | sample_1 | isotropic | sample_conditions_1 |
| (HB)CB(CGCDCE)HE | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CcpNMR, CCPN - chemical shift assignment, data analysis, peak picking
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Varian VNS 600 MHz
- Varian VNS 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB23383 BAE26880 BAG36949 BAG59309 BAG61627 |
| EMBL | CAB66521 CAG33337 CAG38587 CAH93100 CAH93223 |
| GB | AAA75595 AAC13359 AAF24016 AAG03025 AAH50358 |
| REF | NP_001004199 NP_001073333 NP_001126895 NP_001128892 NP_001193830 |
| SP | Q3UKC1 Q5R4U3 Q66HA4 Q86VP1 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts