BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19376

Title: Calmodulin, C-terminal domain, M144H mutant

Deposition date: 2013-07-18 Original release date: 2014-06-16

Authors: Moroz, Yurii; Wu, Yibing; van Nuland, Nicolaas; Korendovych, Ivan

Citation: Moroz, Yurii; Wu, Yibing; van Nuland, Nicolaas; Korendovych, Ivan. "Calmodulin, C-terminal domain, M144H mutant"  Not known ., .-..

Assembly members:
Calmodulin, C-terminal domain, polymer, 75 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Calmodulin, C-terminal domain: SLMKDTDSEEEIREAFRVFD KDGNGYISAAELRHVMTNLG EKLTDEEVDEMIREADIDGD GQVNYEEFVQHMTAK

Data sets:
Data typeCount
13C chemical shifts319
15N chemical shifts78
1H chemical shifts512

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin, C-terminal domain1
2calcium ion2

Entities:

Entity 1, Calmodulin, C-terminal domain 75 residues - Formula weight is not available

1   SERLEUMETLYSASPTHRASPSERGLUGLU
2   GLUILEARGGLUALAPHEARGVALPHEASP
3   LYSASPGLYASNGLYTYRILESERALAALA
4   GLULEUARGHISVALMETTHRASNLEUGLY
5   GLULYSLEUTHRASPGLUGLUVALASPGLU
6   METILEARGGLUALAASPILEASPGLYASP
7   GLYGLNVALASNTYRGLUGLUPHEVALGLN
8   HISMETTHRALALYS

Entity 2, calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Calmodulin, C-terminal domain, [U-100% 13C; U-100% 15N], 1.1 mM; sodium azide 0.01%; sodium chloride 100 mM; calcium chloride 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR, Varian - collection

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

PSVS, Bhattacharya and Montelione - data analysis

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

BMRB 16994 17881 17981 17982 17983 18084 18323 25344
PDB
DBJ BAC39089 BAF45809 BAI66109 BAI66110 BAI66111
EMBL CAB51566
GB AAH10730 AAO17827 AAQ14324 AAX61134 AAY87961
REF XP_001687795 XP_001869425 XP_007442524 XP_009577260 XP_010190430

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts