BMRB Entry 19392
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19392
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Title: Human Bcl10 CARD PubMed: 24074955
Deposition date: 2013-07-26 Original release date: 2013-10-14
Authors: Zheng, Chao; Bracken, Clay; Wu, Hao
Citation: Qiao, Qi; Yang, Chenghua; Zheng, Chao; Fontan, Lorena; David, Liron; Yu, Xiong; Bracken, Clay; Rosen, Monica; Melnick, Ari; Egelman, Edward; Wu, Hao. "Structural Architecture of the CARMA1/Bcl10/MALT1 Signalosome: Nucleation-Induced Filamentous Assembly" Mol. Cell 51, 766-779 (2013).
Assembly members:
Bcl10, polymer, 118 residues, 12642.762 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Bcl10: GSHMEPTAPSLTEEDLTEVK
KDALENLRVYLCEKIIAERH
FDHLRAKKILSREDTREISC
RTSSRKRAGKLLDYLQENPK
GLDTLVESIRREKTQNFLIQ
KITDEVLKLRNIKLEHLK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 466 |
| 15N chemical shifts | 116 |
| 1H chemical shifts | 777 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Bcl10_1 | 1 |
| 2 | Bcl10_2 | 1 |
| 3 | Bcl10_3 | 1 |
| 4 | Bcl10_4 | 1 |
| 5 | Bcl10_5 | 1 |
| 6 | Bcl10_6 | 1 |
| 7 | Bcl10_7 | 1 |
| 8 | Bcl10_8 | 1 |
| 9 | Bcl10_9 | 1 |
| 10 | Bcl10_10 | 1 |
| 11 | Bcl10_11 | 1 |
| 12 | Bcl10_12 | 1 |
| 13 | Bcl10_13 | 1 |
| 14 | Bcl10_14 | 1 |
| 15 | Bcl10_15 | 1 |
| 16 | Bcl10_16 | 1 |
| 17 | Bcl10_17 | 1 |
| 18 | Bcl10_18 | 1 |
| 19 | Bcl10_19 | 1 |
| 20 | Bcl10_20 | 1 |
Entities:
Entity 1, Bcl10_1 118 residues - 12642.762 Da.
| 1 | GLY | SER | HIS | MET | GLU | PRO | THR | ALA | PRO | SER | ||||
| 2 | LEU | THR | GLU | GLU | ASP | LEU | THR | GLU | VAL | LYS | ||||
| 3 | LYS | ASP | ALA | LEU | GLU | ASN | LEU | ARG | VAL | TYR | ||||
| 4 | LEU | CYS | GLU | LYS | ILE | ILE | ALA | GLU | ARG | HIS | ||||
| 5 | PHE | ASP | HIS | LEU | ARG | ALA | LYS | LYS | ILE | LEU | ||||
| 6 | SER | ARG | GLU | ASP | THR | ARG | GLU | ILE | SER | CYS | ||||
| 7 | ARG | THR | SER | SER | ARG | LYS | ARG | ALA | GLY | LYS | ||||
| 8 | LEU | LEU | ASP | TYR | LEU | GLN | GLU | ASN | PRO | LYS | ||||
| 9 | GLY | LEU | ASP | THR | LEU | VAL | GLU | SER | ILE | ARG | ||||
| 10 | ARG | GLU | LYS | THR | GLN | ASN | PHE | LEU | ILE | GLN | ||||
| 11 | LYS | ILE | THR | ASP | GLU | VAL | LEU | LYS | LEU | ARG | ||||
| 12 | ASN | ILE | LYS | LEU | GLU | HIS | LEU | LYS |
Samples:
sample_1: Bcl10, [U-13C; U-15N], 0.3 – 0.4 mM; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR, CCPN - chemical shift assignment
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Varian Unity 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA88822 BAF84035 BAI45655 |
| EMBL | CAA06955 |
| GB | AAC99767 AAD15800 AAD16428 AAD24918 AAD32597 |
| REF | NP_001071496 NP_001096683 NP_001248144 NP_003912 NP_112618 |
| SP | O95999 Q9QYN5 |
| TPG | DAA31335 |
Download simulated HSQC data in one of the following formats:
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