BMRB Entry 19498

Name: gp1.2
Published: 2016-04-18

Click here to enlarge.

PDB ID: 2mdp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19498
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: gp1.2

Deposition date: 2013-09-16 Original release date: 2016-04-18

Authors: Mueller, Geoffrey; London, Robert; Schaaper, Roel

Citation: Singh, Deepa. "The bacteriophage T7 encoded inhibitor (gp1.2) of E. coli dGTP triphosphohydrolase" Not known ., .-..

Assembly members:
entity, polymer, 170 residues, 20389.061 Da.

Natural source: Common Name: Bacteriophage T7 Taxonomy ID: 10760 Superkingdom: Viruses Kingdom: not available Genus/species: Bacteriophage T7

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MGRLYSGNLAAFKAATNKLF QLDLAVIYDDWYDAYTRKDC IRLRIEDRSGNLIDTSTFYH HDEDVLFNMCTDWLNHMYDQ LKDWKMGRLYSGNLAAFKAA TNKLFQLDLAVIYDDWYDAY TRKDCIRLRIEDRSGNLIDT STFYHHDEDVLFNMCTDWLN HMYDQLKDWK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	266
15N chemical shifts	75
1H chemical shifts	488

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	bacteriophage T7 encoded inhibitor (gp1.2)	1

Entities:

Entity 1, bacteriophage T7 encoded inhibitor (gp1.2) 170 residues - 20389.061 Da.

1	MET	GLY	ARG	LEU	TYR	SER	GLY	ASN	LEU	ALA
2	ALA	PHE	LYS	ALA	ALA	THR	ASN	LYS	LEU	PHE
3	GLN	LEU	ASP	LEU	ALA	VAL	ILE	TYR	ASP	ASP
4	TRP	TYR	ASP	ALA	TYR	THR	ARG	LYS	ASP	CYS
5	ILE	ARG	LEU	ARG	ILE	GLU	ASP	ARG	SER	GLY
6	ASN	LEU	ILE	ASP	THR	SER	THR	PHE	TYR	HIS
7	HIS	ASP	GLU	ASP	VAL	LEU	PHE	ASN	MET	CYS
8	THR	ASP	TRP	LEU	ASN	HIS	MET	TYR	ASP	GLN
9	LEU	LYS	ASP	TRP	LYS	MET	GLY	ARG	LEU	TYR
10	SER	GLY	ASN	LEU	ALA	ALA	PHE	LYS	ALA	ALA
11	THR	ASN	LYS	LEU	PHE	GLN	LEU	ASP	LEU	ALA
12	VAL	ILE	TYR	ASP	ASP	TRP	TYR	ASP	ALA	TYR
13	THR	ARG	LYS	ASP	CYS	ILE	ARG	LEU	ARG	ILE
14	GLU	ASP	ARG	SER	GLY	ASN	LEU	ILE	ASP	THR
15	SER	THR	PHE	TYR	HIS	HIS	ASP	GLU	ASP	VAL
16	LEU	PHE	ASN	MET	CYS	THR	ASP	TRP	LEU	ASN
17	HIS	MET	TYR	ASP	GLN	LEU	LYS	ASP	TRP	LYS

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.2 mM; sodium chloride 46 mM; potassium chloride 1 mM; sodium phosphate 1.4 mM; potassium phosphate 0.05 mM; H2O 90%; D2O 10%

sample_1plusphage: entity, [U-100% 13C; U-100% 15N], 0.2 mM; sodium chloride 46 mM; potassium chloride 1 mM; sodium phosphate 1.4 mM; potassium phosphate 0.05 mM; Pf1 phage 50 mg/mL; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 69.8 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	anisotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

CS-Rosetta, Shen, Vernon, Baker and Bax - data analysis

CYANA, Herrmann, Guntert and Wuthrich - structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	MET	GLY	ARG	LEU	TYR	SER	GLY	ASN	LEU	ALA
2	ALA	PHE	LYS	ALA	ALA	THR	ASN	LYS	LEU	PHE
3	GLN	LEU	ASP	LEU	ALA	VAL	ILE	TYR	ASP	ASP
4	TRP	TYR	ASP	ALA	TYR	THR	ARG	LYS	ASP	CYS
5	ILE	ARG	LEU	ARG	ILE	GLU	ASP	ARG	SER	GLY
6	ASN	LEU	ILE	ASP	THR	SER	THR	PHE	TYR	HIS
7	HIS	ASP	GLU	ASP	VAL	LEU	PHE	ASN	MET	CYS
8	THR	ASP	TRP	LEU	ASN	HIS	MET	TYR	ASP	GLN
9	LEU	LYS	ASP	TRP	LYS	MET	GLY	ARG	LEU	TYR
10	SER	GLY	ASN	LEU	ALA	ALA	PHE	LYS	ALA	ALA
11	THR	ASN	LYS	LEU	PHE	GLN	LEU	ASP	LEU	ALA
12	VAL	ILE	TYR	ASP	ASP	TRP	TYR	ASP	ALA	TYR
13	THR	ARG	LYS	ASP	CYS	ILE	ARG	LEU	ARG	ILE
14	GLU	ASP	ARG	SER	GLY	ASN	LEU	ILE	ASP	THR
15	SER	THR	PHE	TYR	HIS	HIS	ASP	GLU	ASP	VAL
16	LEU	PHE	ASN	MET	CYS	THR	ASP	TRP	LEU	ASN
17	HIS	MET	TYR	ASP	GLN	LEU	LYS	ASP	TRP	LYS

1	MET	GLY	ARG	LEU	TYR	SER	GLY	ASN	LEU	ALA
2	ALA	PHE	LYS	ALA	ALA	THR	ASN	LYS	LEU	PHE
3	GLN	LEU	ASP	LEU	ALA	VAL	ILE	TYR	ASP	ASP
4	TRP	TYR	ASP	ALA	TYR	THR	ARG	LYS	ASP	CYS
5	ILE	ARG	LEU	ARG	ILE	GLU	ASP	ARG	SER	GLY
6	ASN	LEU	ILE	ASP	THR	SER	THR	PHE	TYR	HIS
7	HIS	ASP	GLU	ASP	VAL	LEU	PHE	ASN	MET	CYS
8	THR	ASP	TRP	LEU	ASN	HIS	MET	TYR	ASP	GLN
9	LEU	LYS	ASP	TRP	LYS	MET	GLY	ARG	LEU	TYR
10	SER	GLY	ASN	LEU	ALA	ALA	PHE	LYS	ALA	ALA
11	THR	ASN	LYS	LEU	PHE	GLN	LEU	ASP	LEU	ALA
12	VAL	ILE	TYR	ASP	ASP	TRP	TYR	ASP	ALA	TYR
13	THR	ARG	LYS	ASP	CYS	ILE	ARG	LEU	ARG	ILE
14	GLU	ASP	ARG	SER	GLY	ASN	LEU	ILE	ASP	THR
15	SER	THR	PHE	TYR	HIS	HIS	ASP	GLU	ASP	VAL
16	LEU	PHE	ASN	MET	CYS	THR	ASP	TRP	LEU	ASN
17	HIS	MET	TYR	ASP	GLN	LEU	LYS	ASP	TRP	LYS

1	MET	GLY	ARG	LEU	TYR	SER	GLY	ASN	LEU	ALA
2	ALA	PHE	LYS	ALA	ALA	THR	ASN	LYS	LEU	PHE
3	GLN	LEU	ASP	LEU	ALA	VAL	ILE	TYR	ASP	ASP
4	TRP	TYR	ASP	ALA	TYR	THR	ARG	LYS	ASP	CYS
5	ILE	ARG	LEU	ARG	ILE	GLU	ASP	ARG	SER	GLY
6	ASN	LEU	ILE	ASP	THR	SER	THR	PHE	TYR	HIS
7	HIS	ASP	GLU	ASP	VAL	LEU	PHE	ASN	MET	CYS
8	THR	ASP	TRP	LEU	ASN	HIS	MET	TYR	ASP	GLN
9	LEU	LYS	ASP	TRP	LYS	MET	GLY	ARG	LEU	TYR
10	SER	GLY	ASN	LEU	ALA	ALA	PHE	LYS	ALA	ALA
11	THR	ASN	LYS	LEU	PHE	GLN	LEU	ASP	LEU	ALA
12	VAL	ILE	TYR	ASP	ASP	TRP	TYR	ASP	ALA	TYR
13	THR	ARG	LYS	ASP	CYS	ILE	ARG	LEU	ARG	ILE
14	GLU	ASP	ARG	SER	GLY	ASN	LEU	ILE	ASP	THR
15	SER	THR	PHE	TYR	HIS	HIS	ASP	GLU	ASP	VAL
16	LEU	PHE	ASN	MET	CYS	THR	ASP	TRP	LEU	ASN
17	HIS	MET	TYR	ASP	GLN	LEU	LYS	ASP	TRP	LYS