BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 195

Title: Stereospecific Nuclear Magnetic Resonance Assignments of the Methyl Groups of Valine and Leucine in the DNA-Binding Domain of the 434 Repressor by Biosynthetically Directed Fractional 13C Labeling

Deposition date: 1995-07-31 Original release date: 1995-07-31

Authors: Neri, Dario; Szyperski, Thomas; Otting, Gottfried; Senn, Hans; Wuthrich, Kurt

Citation: Neri, Dario; Szyperski, Thomas; Otting, Gottfried; Senn, Hans; Wuthrich, Kurt. "Stereospecific Nuclear Magnetic Resonance Assignments of the Methyl Groups of Valine and Leucine in the DNA-Binding Domain of the 434 Repressor by Biosynthetically Directed Fractional 13C Labeling"  Biochemistry 28, 7510-7516 (1989).

Assembly members:
434 repressor, polymer, 69 residues, Formula weight is not available

Natural source:   Common Name: Escherichia coli bacteriophage   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: not available   Host organism: Escherichia coli

Entity Sequences (FASTA):
434 repressor: SISSRVKSKRIQLGLNQAEL AQKVGTTQQSIEQLENGKTK RPRFLPELASALGVSVDWLL NGTSDSNVR

Data sets:
Data typeCount
13C chemical shifts28
1H chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1434 repressor1

Entities:

Entity 1, 434 repressor 69 residues - Formula weight is not available

1   SERILESERSERARGVALLYSSERLYSARG
2   ILEGLNLEUGLYLEUASNGLNALAGLULEU
3   ALAGLNLYSVALGLYTHRTHRGLNGLNSER
4   ILEGLUGLNLEUGLUASNGLYLYSTHRLYS
5   ARGPROARGPHELEUPROGLULEUALASER
6   ALALEUGLYVALSERVALASPTRPLEULEU
7   ASNGLYTHRSERASPSERASNVALARG

Samples:

sample_one:

sample_condition_set_one: pH: 6 na; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 2539
PDB
DBJ BAT39846
EMBL CAA68301 CBG33631 CSZ37563 CTR12671 CTR54377
GB AAA72530 AAD50896 ABF02546 ADD54938 AEZ38914
PIR S32822
PRF 1011238A
REF WP_000028392 WP_000028393 WP_000028394 WP_000028395 WP_001592290
SP P16117