BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19539

Title: 1H, 13C, 15N chemical shifts assignments of streptomyces virginiae VirA acp5b

Deposition date: 2013-10-07 Original release date: 2014-06-02

Authors: Davison, Jack; Dorival, Jonathan; Rabeharindranto, M; Mazon, Hortense; Chagot, Benjamin; Gruez, Arnaud; Weissman, Kira

Citation: Davison, Jack; Dorival, Jonathan; Rabeharindranto, M; Mazon, Hortense; Chagot, Benjamin; Gruez, Arnaud; Weissman, Kira. "1H, 13C, 15N chemical shifts assignments of streptomyces virginiae VirA acp5b"  Not known ., .-..

Assembly members:
acp5b, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: Streptomyces virginiae   Taxonomy ID: 1961   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces virginiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
acp5b: GPGSAVAVDPAPVARALREE LARTLYCEPGDIDDEASFNT LGLDSILGVEFVAFVNQTYG LDEKAGILYDHPSLAALSRH VAGRAAPV

Data sets:
Data typeCount
13C chemical shifts357
15N chemical shifts93
1H chemical shifts591

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1acp5b1

Entities:

Entity 1, acp5b 88 residues - Formula weight is not available

1   GLYPROGLYSERALAVALALAVALASPPRO
2   ALAPROVALALAARGALALEUARGGLUGLU
3   LEUALAARGTHRLEUTYRCYSGLUPROGLY
4   ASPILEASPASPGLUALASERPHEASNTHR
5   LEUGLYLEUASPSERILELEUGLYVALGLU
6   PHEVALALAPHEVALASNGLNTHRTYRGLY
7   LEUASPGLULYSALAGLYILELEUTYRASP
8   HISPROSERLEUALAALALEUSERARGHIS
9   VALALAGLYARGALAALAPROVAL

Samples:

sample_1: acp5b, [U-99% 13C; U-99% 15N], 1 mM; EDTA 1 mM; sodium phosphate 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY, Goddard - data analysis

TOPSPIN, Bruker Biospin - collection

TALOS+, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts