BMRB Entry 19789

Name: N domain of cardiac troponin C bound to the switch fragment of fast skeletal troponin I at pH 6
Published: 2014-02-11

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PDB ID: 2mkp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19789
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: N domain of cardiac troponin C bound to the switch fragment of fast skeletal troponin I at pH 6 PubMed: 24333682

Deposition date: 2014-02-11 Original release date: 2014-02-11

Authors: Robertson, Ian; Pineda-Sanabria, Sandra; Holmes, Peter; Sykes, Brian

Citation: Robertson, Ian; Pineda-Sanabria, Sandra; Holmes, Peter; Sykes, Brian. "Conformation of the critical pH sensitive region of troponin depends upon a single residue in troponin I." Arch. Biochem. Biophys. ., .-. (2013).

Assembly members:
cNTnC, polymer, 89 residues, 10070.361 Da.
sTnI(115-131), polymer, 17 residues, 1862.297 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
cNTnC: MDDIYKAAVEQLTEEQKNEF KAAFDIFVLGAEDGCISTKE LGKVMRMLGQNPTPEELQEM IDEVDEDGSGTVDFDEFLVM MVRCMKDDS
sTnI(115-131): RMSADAMLKALLGSKHK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	94
15N chemical shifts	82
1H chemical shifts	416

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	cNTnC	1
2	sTnI(115-131)	2
3	entity_CA	3

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Related Database Links:

BMRB	15385 15388 15400 15427 16190 16752 17103 25034 25035 25120 25495 4401
PDB	1AJ4 1AP4 1DTL 1J1D 1J1E 1LA0 1LXF 1MXL 1SPY 1WRK 1WRL 2CTN 2JT0 2JT3 2JT8 2JTZ 2JXL 2KFX 2KGB 2KRD 2L1R 2MKP 2MZP 3SD6 3SWB 4GJE 4GJF 2MKP
DBJ	BAA02369 BAG36483
EMBL	CAA30736 CAG46663 CAG46683 CAA32392 CAB59981 CAF89916 CAF89918 CAF89919
GB	AAA36772 AAA37492 AAA37493 AAA48654 AAA49502 AAA19813 AAA31490 AAA40485 AAA42149 AAB26824
PIR	S07450 TPHUCC
PRF	1403394A 1510257A 750650A 1914142A
REF	NP_001029277 NP_001029523 NP_001123715 NP_001272501 NP_001291793 NP_001027530 NP_001076252 NP_001139301 NP_001139313 NP_001162377
SP	P02591 P05936 P09860 P19123 P63315 P02643 P13412 P27768 P48788
TPG	DAA16908 DAA13498

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Biological Magnetic Resonance Data Bank

BMRB Entry 19789

Title: N domain of cardiac troponin C bound to the switch fragment of fast skeletal troponin I at pH 6 PubMed: 24333682

Additional metadata:

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