BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19797

Title: STRUCTURE OF THE NA,K-ATPASE REGULATORY PROTEIN FXYD2b IN MICELLES   PubMed: 24794573

Deposition date: 2014-02-13 Original release date: 2014-05-12

Authors: Marassi, Francesca; Gong, Xiao-Min

Citation: Gong, Xiao-Min; Marassi, Francesca. "Structure of the Na,K-ATPase regulatory protein FXYD2b in micelles: Implications for membrane-water interfacial arginines"  Biochim. Biophys. Acta ., .-..

Assembly members:
entity, polymer, 64 residues, 7331.390 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: LDRWYLGGSPKGDVDPFYYD YETVRNGGLIFAGLAFIVGL LILLSRRFRSGGNKKRRQIN EDEP

Data sets:
Data typeCount
13C chemical shifts169
15N chemical shifts60
1H chemical shifts229

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FXYD2b1

Entities:

Entity 1, FXYD2b 64 residues - 7331.390 Da.

1   LEUASPARGTRPTYRLEUGLYGLYSERPRO
2   LYSGLYASPVALASPPROPHETYRTYRASP
3   TYRGLUTHRVALARGASNGLYGLYLEUILE
4   PHEALAGLYLEUALAPHEILEVALGLYLEU
5   LEUILELEULEUSERARGARGPHEARGSER
6   GLYGLYASNLYSLYSARGARGGLNILEASN
7   GLUASPGLUPRO

Samples:

sample_1: FXYD2b, [U-99% 13C; U-99% 15N], 0.2 mM; SDS micelles 500 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 50 mM; pH: 5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1anisotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAG34359 AAG34361 AAG37906 AAH13289 AAX36673
REF NP_001671
SP P54710

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts