BMRB Entry 19800
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19800
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Title: Structure of the PrgK first periplasmic domain
Deposition date: 2014-02-14 Original release date: 2014-10-27
Authors: Bergeron, Julien; McIntosh, Lawrence; Strynadka, Natalie
Citation: Bergeron, Julien; Worrall, Liam; De, Soumya; Sgourakis, Nikolaos; Cheung, Adrienne; Lameignere, Emilie; Okon, Mark; Baker, David; McIntosh, Lawrence; Strynadka, Natalie. "The structure of PrgK reveals structural rearrangements upon assembly of the type III secretion system basal body." Structure ., .-..
Assembly members:
entity, polymer, 58 residues, 6532.430 Da.
Natural source: Common Name: Salmonella typhimurium Taxonomy ID: 90371 Superkingdom: Bacteria Kingdom: not available Genus/species: Salmonella typhimurium
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity: KDKDLLKGLDQEQANEVIAV
LQMHNIEANKIDSGKLGYSI
TVAEPDFTAAVYWIKTYQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 250 |
| 15N chemical shifts | 57 |
| 1H chemical shifts | 400 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PrgK first periplasmic domain | 1 |
Entities:
Entity 1, PrgK first periplasmic domain 58 residues - 6532.430 Da.
| 1 | LYS | ASP | LYS | ASP | LEU | LEU | LYS | GLY | LEU | ASP | ||||
| 2 | GLN | GLU | GLN | ALA | ASN | GLU | VAL | ILE | ALA | VAL | ||||
| 3 | LEU | GLN | MET | HIS | ASN | ILE | GLU | ALA | ASN | LYS | ||||
| 4 | ILE | ASP | SER | GLY | LYS | LEU | GLY | TYR | SER | ILE | ||||
| 5 | THR | VAL | ALA | GLU | PRO | ASP | PHE | THR | ALA | ALA | ||||
| 6 | VAL | TYR | TRP | ILE | LYS | THR | TYR | GLN |
Samples:
sample_1: PrgK, [U-100% 13C; U-100% 15N], 1 mM; D2O 10%; H2O 90%; HEPES 25 mM
sample_conditions_1: ionic strength: 0 M; pH: 6.8; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, G??ntert P. - refinement
NMR spectrometers:
- Bruker Avance 850 MHz
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAJ37863 BAP08776 |
| EMBL | CAD05978 CAR34291 CAR38583 CAR60779 CBG25840 |
| GB | AAB60191 AAL21751 AAO70334 AAV78586 AAX66709 |
| PIR | AC0849 |
| REF | NP_457265 NP_461792 WP_000001630 WP_000621236 WP_000621237 |
| SP | P41786 |
Download simulated HSQC data in one of the following formats:
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