BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19813

Title: Solution structure of BmKTX-D19K/K6D

Deposition date: 2014-02-24 Original release date: 2015-02-23

Authors: Hong, Jing; Lin, Donghai; Chen, Zongyun; Wu, Yingliang

Citation: Hong, Jing; Lin, Donghai; Chen, Zongyun; Wu, Yingliang. "Solution structure of BmKTX-D19K/K6D"  Not known ., .-..

Assembly members:
entity, polymer, 37 residues, 3977.839 Da.

Natural source:   Common Name: Manchurian scorpion   Taxonomy ID: 34649   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesobuthus martensii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: VGINVDCKHSGQCLKPCKKA GMRFGKCINGKCDCTPK

Data sets:
Data typeCount
1H chemical shifts240

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 37 residues - 3977.839 Da.

1   VALGLYILEASNVALASPCYSLYSHISSER
2   GLYGLNCYSLEULYSPROCYSLYSLYSALA
3   GLYMETARGPHEGLYLYSCYSILEASNGLY
4   LYSCYSASPCYSTHRPROLYS

Samples:

sample_1: entity 2 mM; D2O, [U-99% 2H], 10%; H2O 90%; potassium phosphate 25 mM

sample_conditions_1: ionic strength: 0.025 M; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O, . - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

SPARKY, Goddard - peak picking

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

BMRB 19810
PDB