BMRB Entry 19815
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19815
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Title: NMR Assignment of Coiled Coil Domain of Myosin Binding Subunit of Myosin Light Chain Phosphatase PubMed: 24693955
Deposition date: 2014-02-24 Original release date: 2014-03-24
Authors: SHARMA, ALOK; RIGBY, ALAN
Citation: Sharma, Alok; Rigby, Alan. "NMR Assignment and Secondary Structure of Coiled Coil Domain of C-terminal Myosin Binding Subunit of Myosin Phosphatase." Protein Pept. Lett. 21, 639-645 (2014).
Assembly members:
COILED_COIL_DOMAIN_OF_MLCP, polymer, 50 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
COILED_COIL_DOMAIN_OF_MLCP: DFKKLYEQILAENEKLKAQL
HDTNMELTDLKLQLEKATQR
QERFADRSLL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 346 |
| 13C chemical shifts | 206 |
| 15N chemical shifts | 50 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SUBUNIT 12A, 1 | 1 |
| 2 | SUBUNIT 12A, 2 | 1 |
Entities:
Entity 1, SUBUNIT 12A, 1 50 residues - Formula weight is not available
| 1 | ASP | PHE | LYS | LYS | LEU | TYR | GLU | GLN | ILE | LEU | |
| 2 | ALA | GLU | ASN | GLU | LYS | LEU | LYS | ALA | GLN | LEU | |
| 3 | HIS | ASP | THR | ASN | MET | GLU | LEU | THR | ASP | LEU | |
| 4 | LYS | LEU | GLN | LEU | GLU | LYS | ALA | THR | GLN | ARG | |
| 5 | GLN | GLU | ARG | PHE | ALA | ASP | ARG | SER | LEU | LEU |
Samples:
sample_1: COILED COIL DOMAIN OF MLCP, [U-13C; U-15N], 1.0 mM; H2O 92%; D2O 8%
sample_conditions_1: ionic strength: 0.01 M; pH: 7.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA07201 BAA07202 BAA22378 BAB23563 BAC28990 |
| EMBL | CAH91185 |
| GB | AAB32730 AAB32731 AAI11753 AAI25382 AAI37631 |
| REF | NP_001137357 NP_001137358 NP_001231921 NP_001253022 NP_002471 |
| SP | O14974 Q10728 Q90623 Q9DBR7 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts