BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19824

Title: Solution structure of a TrkAIg2 domain construct for use in drug discovery

Deposition date: 2014-02-28 Original release date: 2015-01-12

Authors: Shoemark, D.; Fahey, M.; Williams, C.; Sessions, R.; Crump, Matthew; Allen-birt, S.; A., S.

Citation: Allen, S.; Watson, J.; Shoemark, D.; Barua, N.; Patel, N.. "GDNF, NGF and BDNF as therapeutic options for neurodegeneration."  Pharmacology & Therapeutics ., .-..

Assembly members:
HIGH_AFFINITY_NERVE_GROWTH_FACTOR_RECEPTOR, polymer, 107 residues, 11776.0322 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HIGH_AFFINITY_NERVE_GROWTH_FACTOR_RECEPTOR: DDDDDVSFCASVQLHTAVEM HHWCIPFSVDGQPAPSLRWL FNGSVLNETSFIFTEFLEPA ANETVRHGCLRLNQPTHVNN GNYTLLAANPCGQASASIMA AFMDNPF

Data sets:
Data typeCount
13C chemical shifts441
15N chemical shifts113
1H chemical shifts690

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HIGH AFFINITY NERVE GROWTH FACTOR RECEPTOR1

Entities:

Entity 1, HIGH AFFINITY NERVE GROWTH FACTOR RECEPTOR 107 residues - 11776.0322 Da.

1   ASPASPASPASPASPVALSERPHECYSALA
2   SERVALGLNLEUHISTHRALAVALGLUMET
3   HISHISTRPCYSILEPROPHESERVALASP
4   GLYGLNPROALAPROSERLEUARGTRPLEU
5   PHEASNGLYSERVALLEUASNGLUTHRSER
6   PHEILEPHETHRGLUPHELEUGLUPROALA
7   ALAASNGLUTHRVALARGHISGLYCYSLEU
8   ARGLEUASNGLNPROTHRHISVALASNASN
9   GLYASNTYRTHRLEULEUALAALAASNPRO
10   CYSGLYGLNALASERALASERILEMETALA
11   ALAPHEMETASPASNPROPHE

Samples:

sample_1: HIGH AFFINITY NERVE GROWTH FACTOR RECEPTOR, [U-13C; U-15N], 0.75 ± 0.25 mM

sample_conditions_1: ionic strength: 10.000 mM; pH: 6.900; pressure: 1.000 atm; temperature: 293.000 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1solutionsample_conditions_1
TOCSYsample_1solutionsample_conditions_1
HNCAsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
CCONHsample_1solutionsample_conditions_1
HCCH-TOCSYsample_1solutionsample_conditions_1
HCCONHsample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian UnityInova 600 MHz

Related Database Links:

UNP NTRK1_HUMAN
PDB
DBJ BAA34355 BAF83448 BAG35582
GB AAH62580 AAI36555 AAI44240 AHW56542 AHW56543
REF NP_001007793 NP_001012331 NP_002520 XP_001114243 XP_001145942
SP P04629

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts