BMRB Entry 19876

Assembly:

Entities:

Entity 1, YmoB-C117S 133 residues - 14191.914 Da.

Samples:

sample_1: YmoB-C117S 1 mM; sodium phosphate 20 mM; sodium chloride 150 mM; TCEP 1 mM; EDTA 0.2 mM; sodium azide 0.1%; H2O 90%; D2O 10%

sample_2: YmoB-C117S, [U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 150 mM; TCEP 1 mM; EDTA 0.2 mM; sodium azide 0.1%; H2O 90%; D2O 10%

sample_3: YmoB-C117S, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 150 mM; TCEP 1 mM; EDTA 0.2 mM; sodium azide 0.1%; H2O 90%; D2O 10%

sample_4: YmoB-C117S, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 150 mM; TCEP 1 mM; EDTA 0.2 mM; sodium azide 0.1%; H2O 90%; D2O 10%

sample_5: YmoB-C117S, [U-10% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 150 mM; TCEP 1 mM; EDTA 0.2 mM; sodium azide 0.1%; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.00; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

TOPSPIN v2.0 and 3.0, Bruker Biospin - collection

VNMRJ v3.2, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

MddNMR, V. Orekhov, V. Jaravine, M. Mayzel, K. Kazimierczuk, Swedish NMR Center, University of Gothenburg - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

CARA v1.9.0 Beta 3, (c) 2000-2010 by Rochus Keller and others - chemical shift assignment, peak picking

Unio'10 vVersion 2.0.2, 2002-2011 Torsten Herrmann - chemical shift assignment

TALOS v+, Cornilescu, Delaglio and Bax - data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.2.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

ProcheckNMR, Laskowski and MacArthur - data analysis

Molmol, Koradi, Billeter and Wuthrich - data analysis

PyMol, Schr dinger, LLC. - data analysis

NMR spectrometers:

• Bruker DRX 800 MHz
• Varian INOVA 900 MHz
• Varian INOVA 800 MHz