BMRB Entry 19910

Assembly:

Entities:

Entity 1, P22S mutant of N-terminal CS domain of human Shq1 133 residues - 10870.401 Da.

Residues -36 to 0 represent a non-native residues after removal of Gst affinity tag by thrombin

Samples:

sample_1: P22S mutant of human Shq1 CS domain, [U-15N], 0.5 – 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 1 mM; H2O 95%; D2O 5%

sample_2: P22S mutant of human Shq1 CS domain, [U-99% 13C; U-99% 15N], 0.5 – 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 1 mM; H2O 95%; D2O 5%

sample_3: P22S mutant of human Shq1 CS domain, [U-99% 13C; U-99% 15N], 0.5 – 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 1 mM; D2O 100%

sample_conditions_1: temperature: 298 K; pH: 7; pressure: 1 atm; ionic strength: 100 mM

Experiments:

Software:

xwinnmr, Bruker Biospin - collection, processing, data analysis

TOPSPIN, Bruker Biospin - collection, data analysis, processing

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, chemical shift assignment, processing

SPARKY, Goddard - data analysis, chemical shift assignment, chemical shift calculation

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift calculation, data analysis, processing

NMR spectrometers:

• Bruker Avance 800 MHz
• Bruker DRX 600 MHz
• Bruker DRX 500 MHz