BMRB Entry 20103
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20103
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Title: Structural studies of antimicrobial peptide XT-7 and its Lysine4 analogue PubMed: 20116461
Deposition date: 2009-09-24 Original release date: 2010-02-25
Authors: Subasinghage, Anusha; Hewage, Chandralal; Conlon, Michael
Citation: Subasinghage, Anusha; Conlon, Michael; Hewage, Chandralal. "Development of potent anti-infective agents from Silurana tropicalis: Conformational analysis of the amphipathic, alpha-helical antimicrobial peptide XT-7 and its non-haemolytic analogue [G4K]XT-7" Biochim. Biophys. Acta 1804, 1020-1028 (2010).
Assembly members:
[Lysine4]XT-7, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: western clawed frog Taxonomy ID: 8364 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Silurana tropicalis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
[Lysine4]XT-7: GLLKPLLKIAAKVGSNLL
- assigned_chemical_shifts
- spectral_peak_list
Data type | Count |
1H chemical shifts | 128 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | [Lysine4]XT-7 | 1 |
Entities:
Entity 1, [Lysine4]XT-7 18 residues - Formula weight is not available
1 | GLY | LEU | LEU | LYS | PRO | LEU | LEU | LYS | ILE | ALA | ||||
2 | ALA | LYS | VAL | GLY | SER | ASN | LEU | LEU |
Samples:
sample_1: [Lysine4]XT-7 2 mM
sample_conditions_1: pH: 3.2; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
1D | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.11, Goddard - chemical shift assignment, peak picking
CYANA v2.0, Guntert, Mumenthaler and Wuthrich - chemical shift calculation, structure solution
SYBYL v6.8.1, Tripos - refinement
TOPSPIN v2.1, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker DRX 500 MHz