BMRB Entry 20104
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20104
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Title: structural studies of antimicrobial peptide XT-7 and its Lysine mutated peptide PubMed: 20116461
Deposition date: 2009-09-24 Original release date: 2010-02-25
Authors: Subasinghage, Anusha; Hewage, Chandralal; Conlon, Michael
Citation: Subasinghage, Anusha; Conlon, Michael; Hewage, Chandralal. "Development of potent anti-infective agents from Silurana tropicalis: Conformational analysis of the amphipathic, alpha-helical antimicrobial peptide XT-7 and its non-haemolytic analogue [G4K]XT-7" Biochim. Biophys. Acta 1804, 1020-1028 (2010).
Assembly members:
XT-7, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: frog Taxonomy ID: 8364 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Xenopus tropicalis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
XT-7: GLLGPLLKIAAKVGSNLL
- assigned_chemical_shifts
- conformer_family_coord_set
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 116 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | XT-7 peptde | 1 |
Entities:
Entity 1, XT-7 peptde 18 residues - Formula weight is not available
| 1 | GLY | LEU | LEU | GLY | PRO | LEU | LEU | LYS | ILE | ALA | ||||
| 2 | ALA | LYS | VAL | GLY | SER | ASN | LEU | LEU |
Samples:
sample_1: XT-7 2 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 3.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 1D | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.11, Goddard - chemical shift assignment, peak picking
CYANA v2.0, Guntert, Mumenthaler and Wuthrich - chemical shift calculation, structure solution
SYBYL v6.8.1, Tripos - refinement
TOPSPIN v2.1, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker DRX 500 MHz