BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25036

Title: Solution structure of CsUbl

Deposition date: 2014-06-22 Original release date: 2015-06-22

Authors: Ye, Kaiqin

Citation: Ye, Kaiqin. "Solution structure of ubiquitin-like protein from Caldiarchaeum subterraneum"  Not known ., .-..

Assembly members:
entity, polymer, 88 residues, 8107.625 Da.

Natural source:   Common Name: archaea   Taxonomy ID: 311458   Superkingdom: Archaea   Kingdom: not available   Genus/species: Caldiarchaeum subterraneum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: HMKIKIVPAVGGGSPLELEV APNATVGAVRTKVCAMKKLP PDTTRLTYKGRALKDTETLE SLGVADGDKFVLITRTVGGC GEPIRRAA

Data sets:
Data typeCount
13C chemical shifts244
15N chemical shifts80
1H chemical shifts472

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CsUbl1

Entities:

Entity 1, CsUbl 88 residues - 8107.625 Da.

1   HISMETLYSILELYSILEVALPROALAVAL
2   GLYGLYGLYSERPROLEUGLULEUGLUVAL
3   ALAPROASNALATHRVALGLYALAVALARG
4   THRLYSVALCYSALAMETLYSLYSLEUPRO
5   PROASPTHRTHRARGLEUTHRTYRLYSGLY
6   ARGALALEULYSASPTHRGLUTHRLEUGLU
7   SERLEUGLYVALALAASPGLYASPLYSPHE
8   VALLEUILETHRARGTHRVALGLYGLYCYS
9   GLYGLUPROILEARGARGALAALA

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.8 mM; sodium phosphate 1 mM; H2O 90%; D2O 10%

sample_2: entity, [U-100% 15N], 0.8 mM; sodium phosphate 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2solutionsample_conditions_1
3D CBCA(CO)NHsample_1solutionsample_conditions_1
3D C(CO)NHsample_1solutionsample_conditions_1
3D HNCACBsample_1solutionsample_conditions_1
3D HBHA(CO)NHsample_1solutionsample_conditions_1
3D H(CCO)NHsample_1solutionsample_conditions_1
3D 1H-15N NOESYsample_1solutionsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
DBJ BAJ48537 BAJ48615 BAJ49671 BAJ51325

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts