BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25039

Title: Structural Investigation of hnRNP L

Deposition date: 2014-06-24 Original release date: 2015-05-04

Authors: Blatter, Markus; Allain, Frederic

Citation: Blatter, Markus; Allain, Frederic. "Second RNA Recognition Motif Domain of hnRNP L"  To be Published ., .-..

Assembly members:
entity, polymer, 118 residues, 13079.807 Da.

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: QKISRPGDSDDSRSVNSVLL FTILNPIYSITTDVLYTICN PCGPVQRIVIFRKNGVQAMV EFDSVQSAQRAKASLNGADI YSGCCTLKIEYAKPTRLNVF KNDQDTWDYTNPNLSGQG

Data sets:
Data typeCount
13C chemical shifts366
15N chemical shifts129
1H chemical shifts792

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 118 residues - 13079.807 Da.

1   GLNLYSILESERARGPROGLYASPSERASP
2   ASPSERARGSERVALASNSERVALLEULEU
3   PHETHRILELEUASNPROILETYRSERILE
4   THRTHRASPVALLEUTYRTHRILECYSASN
5   PROCYSGLYPROVALGLNARGILEVALILE
6   PHEARGLYSASNGLYVALGLNALAMETVAL
7   GLUPHEASPSERVALGLNSERALAGLNARG
8   ALALYSALASERLEUASNGLYALAASPILE
9   TYRSERGLYCYSCYSTHRLEULYSILEGLU
10   TYRALALYSPROTHRARGLEUASNVALPHE
11   LYSASNASPGLNASPTHRTRPASPTYRTHR
12   ASNPROASNLEUSERGLYGLNGLY

Samples:

sample_1: entity, [U-100% 15N], 1 – 2 mM; sodium chloride 60 mM; sodium phosphate 40 mM; DTT 1 mM; D2O 10%; H2O 90%

sample_2: entity, [U-100% 13C; U-100% 15N], 1 – 2 mM; sodium chloride 60 mM; sodium phosphate 40 mM; DTT 1 mM; D2O 10%; H2O 90%

sample_3: entity, [U-100% 15N], 1 – 2 mM; sodium chloride 60 mM; sodium phosphate 40 mM; DTT 1 mM; D2O 100%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 310.15 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAB18649 BAC39565 BAE02304 BAE26011 BAF84804
EMBL CAA34261
GB AAH27206 AAH69184 AAH99683 AAI69048 EAW56826
PRF 1604358A
REF NP_001005335 NP_001128232 NP_001178959 NP_001252929 NP_001267082
SP P14866 Q8R081
TPG DAA19831

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts