BMRB Entry 25041

Name: Structural Investigation of hnRNP L bound to RNA
Published: 2015-12-21

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PDB ID: 2mqo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25041
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Structural Investigation of hnRNP L bound to RNA

Deposition date: 2014-06-24 Original release date: 2015-12-21

Authors: Blatter, Markus; Allain, Frederic

Citation: Blatter, Markus; Allain, Frederic. "First RNA Recognition Motif Domain of hnRNP L bound to CACACA RNA" To be Published ., .-..

Assembly members:
entity_1, polymer, 105 residues, 11386.674 Da.
RNA_(5'-R(*CP*AP*CP*AP*CP*A)-3'), polymer, 6 residues, 1858.218 Da.

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GENYDDPHKTPASPVVHIRG LIDGVVEADLVEALQEFGPI SYVVVMPKKRQALVEFEDVL GACNAVNYAADNQIYIAGHP AFVNYSTSQKISRPGDSDDS RSVNS
RNA_(5'-R(*CP*AP*CP*AP*CP*A)-3'): CACACA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- shifts2

Data type	Count
13C chemical shifts	363
15N chemical shifts	102
1H chemical shifts	735

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	RNA (5'-R(CPAPCPAPCPA)-3')	2

Entities:

Entity 1, entity_1 105 residues - 11386.674 Da.

1

GLY

GLU

ASN

TYR

ASP

PRO

HIS

LYS

THR

2

PRO

ALA

SER

PRO

VAL

HIS

ILE

ARG

GLY

3

LEU

ILE

ASP

GLY

VAL

GLU

ALA

ASP

LEU

4

VAL

GLU

ALA

LEU

GLN

GLU

PHE

GLY

PRO

ILE

5

SER

TYR

VAL

MET

PRO

LYS

ARG

6

GLN

ALA

LEU

VAL

GLU

PHE

GLU

ASP

VAL

LEU

7

GLY

ALA

CYS

ASN

ALA

VAL

ASN

TYR

ALA

8

ASP

ASN

GLN

ILE

TYR

ILE

ALA

GLY

HIS

PRO

9

ALA

PHE

VAL

ASN

TYR

SER

THR

SER

GLN

LYS

10

ILE

SER

ARG

PRO

GLY

ASP

SER

ASP

SER

11

ARG

SER

VAL

ASN

SER

Entity 2, RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') 6 residues - 1858.218 Da.

1

C

A

C

A

C

A

Samples:

sample_1: entity_1, [U-100% 15N], 2 mM; RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') 2 mM; sodium chloride 60 mM; sodium phosphate 40 mM; DTT 1 mM; D2O 10%; H2O 90%

sample_2: entity_1, [U-100% 13C; U-100% 15N], 2 mM; RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') 2 mM; sodium chloride 60 mM; sodium phosphate 40 mM; DTT 1 mM; D2O 10%; H2O 90%

sample_3: entity_1, [U-100% 15N], 2 mM; RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') 2 mM; sodium chloride 60 mM; sodium phosphate 40 mM; DTT 1 mM; D2O 100%

sample_4: entity_1, [U-100% 13C; U-100% 15N], 2 mM; RNA (5'-R(*CP*AP*CP*AP*CP*A)-3') 2 mM; sodium chloride 60 mM; sodium phosphate 40 mM; DTT 1 mM; D2O 100%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 310.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_3	isotropic	sample_conditions_1
3D F3-filtered-F2-edited NOESY	sample_4	isotropic	sample_conditions_1
2D F2-filtered NOESY	sample_4	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

Bruker Avance 900 MHz
Bruker Avance 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts