BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25096

Title: Solution Structure of MciZ from Bacillus subtilis

Deposition date: 2014-07-16 Original release date: 2015-03-10

Authors: Castellen, Patricia; Sforca, Mauricio; Zeri, Ana; Gueiros-Filho, Frederico

Citation: Bisson-Filho, Alexandre; Discola, Karen; Castellen, Patricia; Blasios, Valdir; Martins, Alexandre; Sforca, Mauricio; Garcia, Wanius; Zeri, Ana; Erickson, Harold; Dessen, Andrea; Gueiros-Filho, Frederico. "Filament capping regulates the bacterial FtsZ cytoskeleton"  Not known ., .-..

Assembly members:
entity, polymer, 40 residues, 4785.832 Da.

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: MKVHRMPKGVVLVGKAWEIR AKLKEYGRTFQYVKDWISKP

Data sets:
Data typeCount
1H chemical shifts263

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MciZ from Bacillus subtilis1

Entities:

Entity 1, MciZ from Bacillus subtilis 40 residues - 4785.832 Da.

1   METLYSVALHISARGMETPROLYSGLYVAL
2   VALLEUVALGLYLYSALATRPGLUILEARG
3   ALALYSLEULYSGLUTYRGLYARGTHRPHE
4   GLNTYRVALLYSASPTRPILESERLYSPRO

Samples:

sample_1: entity 0.2 mM; sodium phosphate 50 mM; potassium chloride 50 mM

sample_2: entity 0.2 mM; sodium phosphate 50 mM; potassium chloride 50 mM

sample_conditions_1: temperature: 298 K; pH: 4.0; pressure: 1 atm; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, chemical shift calculation, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, chemical shift assignment, processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution, refinement

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Agilent INOVA 600 MHz

Related Database Links:

PDB
DBJ BAM52846 BAM58420 BAO93450 GAK81792
EMBL CCQ48600 CCU58870 CEI57579 CEJ78001 CJR81485
GB ADV93099 AEP87211 AEP91387 AFQ58312 AGE64010
REF WP_003230420 WP_014114281 WP_015714212