BMRB Entry 25188

Name: Solution Structure of the YTH Domain of YT521-B in complex with N6-Methyladenosine containing RNA
Published: 2014-11-17

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PDB ID: 2mtv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25188
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution Structure of the YTH Domain of YT521-B in complex with N6-Methyladenosine containing RNA PubMed: 25389274

Deposition date: 2014-09-01 Original release date: 2014-11-17

Authors: Theler, Dominik; Dominguez, Cyril; Blatter, Markus; Boudet, Julien; Allain, Frederic

Citation: Theler, Dominik; Dominguez, Cyril; Blatter, Markus; Boudet, Julien; Allain, Frederic. "Solution structure of the YTH domain in complex with N6-methyladenosine RNA: a reader of methylated RNA" Nucleic Acids Res. 42, 13911-13919 (2014).

Assembly members:
entity_1, polymer, 156 residues, 17508.334 Da.
RNA_(5'-R(*UP*GP*(6MA)P*CP*AP*C)-3'), polymer, 6 residues, 1873.230 Da.

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: QTSKLKSVLQDARFFLIKSN NHENVSLAKAKGVWSTLPVN EKKLNLAFRSARSVILIFSV RESGKFQGFARLSSESHHGG SPIHWVLPAGMSAKMLGGVF KIDWICRRELPFTKSAHLTN PWNEHKPVKIGRDGQEIELE CGTQLCLLFPPDESID
RNA_(5'-R(*UP*GP*(6MA)P*CP*AP*C)-3'): UGXCAC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	683
15N chemical shifts	155
1H chemical shifts	1138

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	RNA (5'-R(UPGP(6MA)PCPAPC)-3')	2

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_3	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H -1H NOESY 13C F1-filtered F2-filtered	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY 13C F1-edited F3-filtered	sample_2	isotropic	sample_conditions_1
2D 1H -1H NOESY 13C15N F2-filtered	sample_1	isotropic	sample_conditions_1

PDB	2MTV 2YUD 4R3H 4R3I
DBJ	BAA23885 BAB71181 BAB85552 BAD32590 BAG10510
EMBL	CAH91339
GB	AAD55973 AAH22697 AAH41119 AAH53863 AAI02815
REF	NP_001026902 NP_001039985 NP_001125792 NP_588611 NP_596914
SP	E9Q5K9 Q96MU7 Q9QY02
TPG	DAA28607

Biological Magnetic Resonance Data Bank

BMRB Entry 25188

Title: Solution Structure of the YTH Domain of YT521-B in complex with N6-Methyladenosine containing RNA PubMed: 25389274

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