BMRB Entry 25194

Name: NMR resonance assignment of the C-terminal domain of the lantibiotic immunity protein NisI
Published: 2015-04-27

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PDB ID: 2n2e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25194
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR resonance assignment of the C-terminal domain of the lantibiotic immunity protein NisI PubMed: 25613223

Deposition date: 2014-09-02 Original release date: 2015-04-27

Authors: Hacker, Carolin; Christ, Nina Alexandra; Duchardt-Ferner, Elke; Bernigner, Lucija; Koetter, Peter; Entian, Karl-Dieter; Woehnert, Jens

Citation: Hacker, Carolin; Christ, Nina Alexandra; Duchardt-Ferner, Elke; Bernigner, Lucija; Koetter, Peter; Entian, Karl-Dieter; Woehnert, Jens. "NMR resonance assignments of the lantibiotic immunity protein NisI from Lactococcus lactis" Biomol NMR Assign. 9, 293-297 (2015).

Assembly members:
NisI, polymer, 130 residues, 14562.9 Da.

Natural source: Common Name: firmicutes Taxonomy ID: 1358 Superkingdom: Bacteria Kingdom: not available Genus/species: Lactococcus lactis

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
NisI: SDKGAVKALRLQNFDVTSDI SDDNFVIDKNDSRKIDYMGN IYSISDTTVSDEELGEYQDV LAEVRVFDSVSGKSIPRSEW GRIDKDGSNSKQSRTEWDYG EIHSIRGKSLTEAFAVEIND DFKLATKVGN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	537
15N chemical shifts	138
1H chemical shifts	836

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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1	SER	ASP	LYS	GLY	ALA	VAL	LYS	ALA	LEU	ARG
2	LEU	GLN	ASN	PHE	ASP	VAL	THR	SER	ASP	ILE
3	SER	ASP	ASP	ASN	PHE	VAL	ILE	ASP	LYS	ASN
4	ASP	SER	ARG	LYS	ILE	ASP	TYR	MET	GLY	ASN
5	ILE	TYR	SER	ILE	SER	ASP	THR	THR	VAL	SER
6	ASP	GLU	GLU	LEU	GLY	GLU	TYR	GLN	ASP	VAL
7	LEU	ALA	GLU	VAL	ARG	VAL	PHE	ASP	SER	VAL
8	SER	GLY	LYS	SER	ILE	PRO	ARG	SER	GLU	TRP
9	GLY	ARG	ILE	ASP	LYS	ASP	GLY	SER	ASN	SER
10	LYS	GLN	SER	ARG	THR	GLU	TRP	ASP	TYR	GLY
11	GLU	ILE	HIS	SER	ILE	ARG	GLY	LYS	SER	LEU
12	THR	GLU	ALA	PHE	ALA	VAL	GLU	ILE	ASN	ASP
13	ASP	PHE	LYS	LEU	ALA	THR	LYS	VAL	GLY	ASN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	15N13C	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	15N13C	isotropic	sample_conditions_1
3D CBCA(CO)NH	15N13C	isotropic	sample_conditions_1
3D HNCO	15N13C	isotropic	sample_conditions_1
3D HNCACB	15N13C	isotropic	sample_conditions_1
3D HBHA(CO)NH	15N13C	isotropic	sample_conditions_1
3D H(CCO)NH	15N13C	isotropic	sample_conditions_1
3D C(CO)NH	15N13C	isotropic	sample_conditions_1
3D 1H-15N NOESY	15N	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	15N13C	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	15N13C	isotropic	sample_conditions_1
3D HNCACO	15N13C	isotropic	sample_conditions_1

BMRB	25193
PDB	2N2E
DBJ	BAL50562
EMBL	CAA54209 CAA79465
GB	AAA25193 AAQ89591 AAQ89592 ACM62695 ADA64984
REF	WP_012897848 WP_014570409 WP_015425983 WP_017864237 WP_039114828
SP	P42708

Biological Magnetic Resonance Data Bank

BMRB Entry 25194

Title: NMR resonance assignment of the C-terminal domain of the lantibiotic immunity protein NisI PubMed: 25613223

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	SER	ASP	LYS	GLY	ALA	VAL	LYS	ALA	LEU	ARG
2	LEU	GLN	ASN	PHE	ASP	VAL	THR	SER	ASP	ILE
3	SER	ASP	ASP	ASN	PHE	VAL	ILE	ASP	LYS	ASN
4	ASP	SER	ARG	LYS	ILE	ASP	TYR	MET	GLY	ASN
5	ILE	TYR	SER	ILE	SER	ASP	THR	THR	VAL	SER
6	ASP	GLU	GLU	LEU	GLY	GLU	TYR	GLN	ASP	VAL
7	LEU	ALA	GLU	VAL	ARG	VAL	PHE	ASP	SER	VAL
8	SER	GLY	LYS	SER	ILE	PRO	ARG	SER	GLU	TRP
9	GLY	ARG	ILE	ASP	LYS	ASP	GLY	SER	ASN	SER
10	LYS	GLN	SER	ARG	THR	GLU	TRP	ASP	TYR	GLY
11	GLU	ILE	HIS	SER	ILE	ARG	GLY	LYS	SER	LEU
12	THR	GLU	ALA	PHE	ALA	VAL	GLU	ILE	ASN	ASP
13	ASP	PHE	LYS	LEU	ALA	THR	LYS	VAL	GLY	ASN

1	SER	ASP	LYS	GLY	ALA	VAL	LYS	ALA	LEU	ARG
2	LEU	GLN	ASN	PHE	ASP	VAL	THR	SER	ASP	ILE
3	SER	ASP	ASP	ASN	PHE	VAL	ILE	ASP	LYS	ASN
4	ASP	SER	ARG	LYS	ILE	ASP	TYR	MET	GLY	ASN
5	ILE	TYR	SER	ILE	SER	ASP	THR	THR	VAL	SER
6	ASP	GLU	GLU	LEU	GLY	GLU	TYR	GLN	ASP	VAL
7	LEU	ALA	GLU	VAL	ARG	VAL	PHE	ASP	SER	VAL
8	SER	GLY	LYS	SER	ILE	PRO	ARG	SER	GLU	TRP
9	GLY	ARG	ILE	ASP	LYS	ASP	GLY	SER	ASN	SER
10	LYS	GLN	SER	ARG	THR	GLU	TRP	ASP	TYR	GLY
11	GLU	ILE	HIS	SER	ILE	ARG	GLY	LYS	SER	LEU
12	THR	GLU	ALA	PHE	ALA	VAL	GLU	ILE	ASN	ASP
13	ASP	PHE	LYS	LEU	ALA	THR	LYS	VAL	GLY	ASN

1	SER	ASP	LYS	GLY	ALA	VAL	LYS	ALA	LEU	ARG
2	LEU	GLN	ASN	PHE	ASP	VAL	THR	SER	ASP	ILE
3	SER	ASP	ASP	ASN	PHE	VAL	ILE	ASP	LYS	ASN
4	ASP	SER	ARG	LYS	ILE	ASP	TYR	MET	GLY	ASN
5	ILE	TYR	SER	ILE	SER	ASP	THR	THR	VAL	SER
6	ASP	GLU	GLU	LEU	GLY	GLU	TYR	GLN	ASP	VAL
7	LEU	ALA	GLU	VAL	ARG	VAL	PHE	ASP	SER	VAL
8	SER	GLY	LYS	SER	ILE	PRO	ARG	SER	GLU	TRP
9	GLY	ARG	ILE	ASP	LYS	ASP	GLY	SER	ASN	SER
10	LYS	GLN	SER	ARG	THR	GLU	TRP	ASP	TYR	GLY
11	GLU	ILE	HIS	SER	ILE	ARG	GLY	LYS	SER	LEU
12	THR	GLU	ALA	PHE	ALA	VAL	GLU	ILE	ASN	ASP
13	ASP	PHE	LYS	LEU	ALA	THR	LYS	VAL	GLY	ASN