BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25268

Title: SpoVM structure determination

Deposition date: 2014-10-06 Original release date: 2015-03-30

Authors: Tian, Fang; Rill, Richard

Citation: Tian, Fang; Rill, Richard; Castaining, Jean-Philippe; Hsin, Jen; Tan, Irene; Wang, Xingshen; Huang, Kerwyn-Casey; Ramamurthi, Kumaran. "Structural and mechanistic basis for the geometric-driven subcellular localication of a small protein"  Nat. Struct. Biol. ., .-..

Assembly members:
Molecule_1, polymer, 26 residues, 3022.7605 Da.

Natural source:   Common Name: firmicutes   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Molecule_1: MKFYTIKLPKFLGGIVRAML GSFRKD

Data sets:
Data typeCount
1H chemical shifts172
13C chemical shifts89
15N chemical shifts22

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 26 residues - 3022.7605 Da.

1   METLYSPHETYRTHRILELYSLEUPROLYS
2   PHELEUGLYGLYILEVALARGALAMETLEU
3   GLYSERPHEARGLYSASP

Samples:

sample_1: SpoVM, [U-100% 13C; U-100% 15N], 0.5 – 1 mM; sodium phosphate 16 mM; sodium chloride 80 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 310 K; pH: 6.5; pressure: 1 atm; ionic strength: 80 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker AvanceII 600 MHz
  • Bruker AvanceIII 850 MHz

Related Database Links:

PDB
EMBL CAA74254 CAB13454
GB AAC36809 AAU23337 AAU40697 ADM37675 ADV96607
REF NP_389463 WP_003221545
SP P37817

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts