BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25273

Title: Solution structure of phosphorylated cytosolic part of Trop2

Deposition date: 2014-10-08 Original release date: 2015-05-04

Authors: Ilc, Gregor; Plavec, Janez; Vidmar, Tilen; Pavsic, Miha; Lenarcic, Brigita

Citation: Ilc, Gregor; Plavec, Janez; Vidmar, Tilen; Pavsic, Miha; Lenarcic, Brigita. "A Structural View of the Trop2-Mediated Signaling Mechanism Revealed by NMR and Molecular Dynamics"  Not known ., .-..

Assembly members:
entity, polymer, 26 residues, 3176.658 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: TNRRKXGKYKKVEIKELGEL RKEPSL

Data sets:
Data typeCount
1H chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 26 residues - 3176.658 Da.

1   THRASNARGARGLYSSEPGLYLYSTYRLYS
2   LYSVALGLUILELYSGLULEUGLYGLULEU
3   ARGLYSGLUPROSERLEU

Samples:

sample_1: Trop2ICP 5 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian VNMRS 800 MHz

Related Database Links:

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