BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25297

Title: Solution-state NMR structure of the lasso peptide streptomonomicin   PubMed: 25601074

Deposition date: 2014-10-24 Original release date: 2015-01-16

Authors: Tietz, Jonathan; Zhu, Lingyang; Mitchell, Douglas; Metelev, Mikhail; Melby, Joel; Blair, Patricia; Livnat, Itamar; Severinov, Konstantin

Citation: Metelev, Mikhail; Tietz, Jonathan; Melby, Joel; Zhu, Lingyang; Blair, Patricia; Livnat, Itamar; Severinov, Konstantin; Mitchell, Douglas. "Structure, bioactivity, and resistance mechanism of streptomonomicin, an unusual lasso Peptide from an understudied halophilic actinomycete"  Chem. Biol. 22, 241-250 (2015).

Assembly members:
entity, polymer, 21 residues, 2252.586 Da.

Natural source:   Common Name: high GC Gram+   Taxonomy ID: 183763   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomonospora alba

Experimental source:   Production method: purified from the natural source   Host organism: Streptomonospora alba

Entity Sequences (FASTA):
entity: SLGSSPYNDILGYPALIVIY P

Data sets:
Data typeCount
13C chemical shifts21
1H chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 21 residues - 2252.586 Da.

1   SERLEUGLYSERSERPROTYRASNASPILE
2   LEUGLYTYRPROALALEUILEVALILETYR
3   PRO

Samples:

sample_1: streptomonomicin 4 mM; methanol 100%

sample_conditions_1: temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HMBCsample_1isotropicsample_conditions_1

Software:

X-PLOR_NIH v2.36, Brunger - refinement, structure solution

SPARKY v3.115, Goddard - peak picking

NMRPipe v8.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ v3.2A, Varian - collection

MestReNova v8.1.1, Mestrelab - chemical shift assignment, processing

NMR spectrometers:

  • Agilent VNMRS 750 MHz

Related Database Links:

PDB
GB KIH99826