BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25539

Title: Three dimensional structure of EPI-X4, a human albumin-derived peptide that regulates innate immunity through the CXCR4/CXCL12 chemotactic axis and antagonizes HIV-1 entry   PubMed: 25921529

Deposition date: 2015-03-18 Original release date: 2015-04-27

Authors: Perez-Castells, Javier; Canales, Angeles; Jimenez-Barbero, Jesus; Gimenez-Gallego, Guillermo

Citation: Zirafi, Onofrio; Kim, Kyeong-ae; Standker, Ludger; Mohr, Katharina B.; Sauter, Daniel; Heigele, Anke; Kluge, Silvia F.; Wiercinska, Eliza; Chudziak, Doreeh; Richter, Rudolf; Moepps, Barbara; Grieschik, Peter; Vas, Virg; Geiger, Hartmut; Lamia, Markus; Weil, Tanja; Burster, Timo; Zgraja, Andreas; Daubeuf, Francois; Forssard, Nelly; Hachet-Haas, Muriel; Heunisch, Fabian; Reichetzeder, Christoph; Galzi, Jean-Luc; Perez-Castells, Javier; Canales, Angeles; Jimenez-Barbero, Jesus; Gimenez-Gallego, Guillermo; Scheneider, Marion; Shorter, James; Telendi, Amalio; Hocher, Berthold; Forssman, Wolf Georg; Kirchhoff, Halvard; Munch, Jan. "Discovery and characterization of an endogenous CXCR4 antagonist"  Cell Rep. 11, 737-747 (2015).

Assembly members:
entity, polymer, 16 residues, 1833.219 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
entity: LVRYTKKVPQVSTPTL

Data sets:
Data typeCount
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 16 residues - 1833.219 Da.

1   LEUVALARGTYRTHRLYSLYSVALPROGLN
2   VALSERTHRPROTHRLEU

Samples:

sample_1: entity 1 mM; sodium phosphate 10 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz