BMRB Entry 25562

Assembly:

Entities:

Entity 1, entity 176 residues - 19169.670 Da.

The recombinant protein contains three extraneous amino acids at the N-terminus and residues 54-226 of human MEL1 (gsgFPTS...GLDE).

Samples:

sample_1: entity, [U-99% 13C; U-99% 15N], 0.45-0.55 mM; potassium phosphate 20 mM; potassium chloride 50 mM; DTT 2.5 mM; H2O 90%; D2O 10%

sample_2: entity, [U-99% 15N], 0.4-0.5 mM; potassium phosphate 20 mM; potassium chloride 50 mM; DTT 2.5 mM; H2O 90%; D2O 10%

sample_3: entity 0.5 mM; potassium phosphate 20 mM; potassium chloride 50 mM; DTT 2.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.088 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

VNMRJ, Agilent - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr, CCPN - chemical shift assignment, data analysis, peak picking

ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

TALOS, Cornilescu, Delaglio and Bax - generation of torsion angle restraints

NMR spectrometers:

• Varian Varian NMR System 800 MHz
• Varian Varian NMR System 900 MHz
• Varian Varian NMR System 500 MHz