BMRB Entry 25645

Assembly:

Entities:

Entity 1, entity_1 98 residues - 10894.250 Da.

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 1.0 mM; sodium phosphate 50 mM; sodium chloride 100 mM; PMSF 1 mM; EDTA 0.1 mM; D2O 10%; H2O 90%

sample_2: entity, [U-100% 13C; U-100% 15N; U-80% 2H], 1.0 mM; sodium phosphate 50 mM; sodium chloride 100 mM; PMSF 1 mM; EDTA 0.1 mM; D2O 10%; H2O 90%

sample_3: entity, [U-100% 13C; U-100% 15N; U-50% 2H], 1.0 mM; sodium phosphate 50 mM; sodium chloride 100 mM; PMSF 1 mM; EDTA 0.1 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 295 K

sample_conditions_2: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 310 K

Experiments:

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

CS-Rosetta, Shen, Vernon, Baker and Bax - structure solution

CcpNMr, CCPN - data analysis

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift calculation

NMR spectrometers:

• Varian INOVA 900 MHz
• Bruker Avance 600 MHz
• Bruker Avance 500 MHz
• Varian INOVA 600 MHz
• Varian INOVA 800 MHz