BMRB Entry 25652
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PDB ID: 2n3o
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25652
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Title: Structure of PTB RRM1(41-163) bound to an RNA stemloop containing a structured loop derived from viral internal ribosomal entry site RNA
Deposition date: 2015-06-08 Original release date: 2016-08-08
Authors: Maris, Christophe; Jayne, Sandrine; Damberger, Fred; Ravindranathan, Sapna; Allain, Frederic
Citation: Maris, Christophe; Jayne, Sandrine; Damberger, Fred; Ravindranathan, Sapna; Allain, Frederic. "C-terminal helix folding upon pyrimidine-rich hairpin binding to PTB RRM1. Implications for PTB function in Encephalomyocarditis virus IRES activity." To be Published ., .-..
Assembly members:
PTBRRM1, polymer, 123 residues, 13375.287 Da.
RNA_(5'-R(*GP*GP*GP*AP*CP*CP*UP*GP*GP*UP*CP*UP*UP*UP*CP*CP*AP*GP*GP*UP*CP*CP*C)-3'), polymer, 23 residues, 7308.393 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PTBRRM1: GNDSKKFKGDSRSAGVPSRV
IHIRKLPIDVTEGEVISLGL
PFGKVTNLLMLKGKNQAFIE
MNTEEAANTMVNYYTSVTPV
LRGQPIYIQFSNHKELKTDS
SPNQARAQAALQAVNSVQSG
NLA
RNA_(5'-R(*GP*GP*GP*AP*CP*CP*UP*GP*GP*UP*CP*UP*UP*UP*CP*CP*AP*GP*GP*UP*CP*CP*C)-3'): GGGACCUGGUCUUUCCAGGU
CCC
- assigned_chemical_shifts
- RDCs
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 535 |
15N chemical shifts | 119 |
1H chemical shifts | 1013 |
residual dipolar couplings | 70 |
Additional metadata:
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