BMRB Entry 25800

Name: Solution structure of Plasmodium falciparum SR1-RRM1 in complex with ACAUCA RNA
Published: 2016-10-06

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PDB ID: 2n7c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25800
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution structure of Plasmodium falciparum SR1-RRM1 in complex with ACAUCA RNA PubMed: 30500338

Deposition date: 2015-09-08 Original release date: 2016-10-06

Authors: Ganguly, Akshay; Verma, Garima; Bhavesh, Neel Sarovar

Citation: Ganguly, Akshay; Verma, Garima; Bhavesh, Neel Sarovar. "The N-terminal RNA Recognition Motif of PfSR1 Confers Semi-specificity for Pyrimidines during RNA Recognition" J. Mol. Biol. ., .-. (2018).

Assembly members:
PfSR1-RRM1, polymer, 89 residues, 9997.284 Da.
RNA_(5'-R(*AP*CP*AP*UP*CP*A)-3'), polymer, 6 residues, 1859.203 Da.

Natural source: Common Name: malaria parasite P. falciparum Taxonomy ID: 5833 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
PfSR1-RRM1: GSHMVIRESVSRIYVGNLPS HVSSRDVENEFRKYGNILKC DVKKTVSGAAFAFIEFEDAR DAADAIKEKDGCDFEGNKLR VEVPFNARE
RNA_(5'-R(*AP*CP*AP*UP*CP*A)-3'): ACAUCA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	398
15N chemical shifts	84
1H chemical shifts	374

Additional metadata:

Assembly
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Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	RNA (5'-R(APCPAPUPCPA)-3')	2

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_H2O	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_H2O	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_H2O	isotropic	sample_conditions_1
3D HNCACB	sample_H2O	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_H2O	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_H2O	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_H2O	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_D2O	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_D2O	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_D2O	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_D2O	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_H2O	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_D2O	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_H2O	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_D2O	isotropic	sample_conditions_1
2D 13C(F1)-filtered, 13C(F2)-filtered 1H-1H NOESY	sample_D2O	isotropic	sample_conditions_1
2D 13C(F2)-edited 1H-1H NOESY aromatic	sample_D2O	isotropic	sample_conditions_1

Biological Magnetic Resonance Data Bank

BMRB Entry 25800

Title: Solution structure of Plasmodium falciparum SR1-RRM1 in complex with ACAUCA RNA PubMed: 30500338

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