BMRB Entry 25831

Assembly:

Entities:

Entity 1, miR-20b 23 residues - 7318.388 Da.

Entity 2, Rbfox_RRM 104 residues - Formula weight is not available

Samples:

sample_1: miR-20b 1 mM; sodium phosphate 10 mM; sodium chloride 20 mM; Rbfox RRM, [U-99% 13C; U-99% 15N], 1 mM; H2O 90%; D2O 10%

sample_2: miR-20b 1 mM; sodium phosphate 10 mM; sodium chloride 20 mM; Rbfox RRM, [U-99% 13C; U-99% 15N], 1 mM; D2O 100%

sample_3: miR-20b, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 10 mM; sodium chloride 20 mM; Rbfox RRM 1 mM; D2O 100%

sample_4: miR-20b, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 10 mM; sodium chloride 20 mM; Rbfox RRM 1 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 279 K

sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

TOPSPIN, Bruker Biospin - collection

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CcpNMR, CCPN - chemical shift assignment, data analysis, peak picking

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

NMR spectrometers:

• Bruker Avance 600 MHz
• Bruker Avance 800 MHz