BMRB Entry 25852
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PDB ID: 2n8j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25852
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Title: Structure and 15N relaxation data of Calmodulin bound to the endothelial Nitric Oxide Synthase Calmodulin Binding Domain Peptide at Physiological Calcium Concentration
Deposition date: 2015-10-16 Original release date: 2016-10-06
Authors: Piazza, Michael; Guillemette, Guy; Dieckmann, Thorsten
Citation: Piazza, Michael; Guillemette, Guy; Dieckmann, Thorsten. "Structure of Calmodulin bound to the endothelial Nitric Oxide Synthase Calmodulin Binding Domain Peptide at Physiological Calcium Concentration" Biochem. Biophys. Res. Commun. ., .-..
Assembly members:
CaM, polymer, 148 residues, 16721.465 Da.
eNOS, polymer, 22 residues, 2385.864 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CaM: ADQLTEEQIAEFKEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGYI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
eNOS: TRKKTFKEVANAVKISASLM
GT
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- order_parameters
| Data type | Count |
| 13C chemical shifts | 415 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 966 |
| T1 relaxation values | 134 |
| T2 relaxation values | 135 |
| heteronuclear NOE values | 146 |
| order parameters | 125 |
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