BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 25910

Title: 1H, 13C, and 15N Chemical Shift Assignments for in-cell GB1   PubMed: 27910948

Deposition date: 2015-11-30 Original release date: 2016-12-21

Authors: Ikeya, Teppei; Hanashima, Tomomi; Hosoya, Saori; Shimazaki, Manato; Ikeda, Shiro; Mishima, Masaki; Guentert, Peter; Ito, Yutaka

Citation: Ikeya, Teppei; Hanashima, Tomomi; Hosoya, Saori; Shimazaki, Manato; Ikeda, Shiro; Mishima, Masaki; Guentert, Peter; Ito, Yutaka. "Improved in-cell structure determination of proteins at near-physiological concentration"  Sci. Rep. 6, 38312-38312 (2016).

Assembly members:
entity, polymer, 57 residues, 6258.896 Da.

Natural source:   Common Name: firmicutes   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MGTYKLILNGKTLKGETTTE AVDAATAEKVFKQYANDNGV DGEWTYDDATKTFTVTE

Data sets:
Data typeCount
13C chemical shifts217
15N chemical shifts57
1H chemical shifts322

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts