BMRB Entry 25956
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25956
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of oxidised RsrA and without zinc ion PubMed: 27432510
Deposition date: 2016-01-15 Original release date: 2016-08-01
Authors: Zdanowski, K.; Pecqueur, L.; Werner, J.; Potts, Jennifer; Kleanthous, C.
Citation: Rajasekar, K.; Zdanowski, K.; Yan, J.; Hopper, J.; Francis, M.; Seepersad, C.; Sharp, C.; Pecqueur, L.; Werner, J.; Robinson, C.; Mohammed, S.; Potts, Jennifer; Kleanthous, C.. "The anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic core" Nat. Commun. 7, 12194-12194 (2016).
Assembly members:
ANTI-SIGMA_FACTOR_RSRA, polymer, 108 residues, 11807.9444 Da.
Natural source: Common Name: Streptomyces coelicolor Taxonomy ID: 1902 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces coelicolor
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
ANTI-SIGMA_FACTOR_RSRA: GSHMSAGEPHETDCSEILDH
LYEFLDKEMPDSDAVKFEHH
FEESSPCLEKYGLEQAVKKL
VKRAAGQDDVPGDLRAKVMG
RLDLIRSGQSVPEHDVAAAP
SSSAPQES
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 327 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 681 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ANTI-SIGMA FACTOR RSRA | 1 |
Entities:
Entity 1, ANTI-SIGMA FACTOR RSRA 108 residues - 11807.9444 Da.
| 1 | GLY | SER | HIS | MET | SER | ALA | GLY | GLU | PRO | HIS | ||||
| 2 | GLU | THR | ASP | CYS | SER | GLU | ILE | LEU | ASP | HIS | ||||
| 3 | LEU | TYR | GLU | PHE | LEU | ASP | LYS | GLU | MET | PRO | ||||
| 4 | ASP | SER | ASP | ALA | VAL | LYS | PHE | GLU | HIS | HIS | ||||
| 5 | PHE | GLU | GLU | SER | SER | PRO | CYS | LEU | GLU | LYS | ||||
| 6 | TYR | GLY | LEU | GLU | GLN | ALA | VAL | LYS | LYS | LEU | ||||
| 7 | VAL | LYS | ARG | ALA | ALA | GLY | GLN | ASP | ASP | VAL | ||||
| 8 | PRO | GLY | ASP | LEU | ARG | ALA | LYS | VAL | MET | GLY | ||||
| 9 | ARG | LEU | ASP | LEU | ILE | ARG | SER | GLY | GLN | SER | ||||
| 10 | VAL | PRO | GLU | HIS | ASP | VAL | ALA | ALA | ALA | PRO | ||||
| 11 | SER | SER | SER | ALA | PRO | GLN | GLU | SER |
Samples:
sample_1: ANTI-SIGMA FACTOR RSRA, [U-13C; U-15N], 1.25 mM
sample_conditions_1: ionic strength: 0.0 mM; pH: 7.100; pressure: 1 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | solution | sample_conditions_1 |
| TOCSY (2D and 3D) HNCA | sample_1 | solution | sample_conditions_1 |
| CBCANH and CBCA(CO)NH | sample_1 | solution | sample_conditions_1 |
| HNCO and HN(CA)CO | sample_1 | solution | sample_conditions_1 |
| HNCA | sample_1 | solution | sample_conditions_1 |
| CBCANH | sample_1 | solution | sample_conditions_1 |
| CBCA(CO)NH | sample_1 | solution | sample_conditions_1 |
| HNCO | sample_1 | solution | sample_conditions_1 |
| HN(CA)CO | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, PDBe - collection
CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts