BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 26056

Title: SOLUTION STRUCTURE OF HELATX1

Deposition date: 2016-05-12 Original release date: 2017-05-30

Authors: PARK, BONG GYU; KIM, JAE IL

Citation: PARK, BONG GYU; KIM, JAE IL. "SOLUTION STRUCTURE OF HELATX1"  Biochim. Biophys. Acta ., .-..

Assembly members:
entity, polymer, 25 residues, 2932.458 Da.

Natural source:   Common Name: scorpions   Taxonomy ID: 217256   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Heterometrus laoticus

Experimental source:   Production method: recombinant technology   Host organism: Synthetic peptide

Entity Sequences (FASTA):
entity: SCKKECSGSRRTKKCMQKCN REHGH

Data sets:
Data typeCount
1H chemical shifts128

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 25 residues - 2932.458 Da.

1   SERCYSLYSLYSGLUCYSSERGLYSERARG
2   ARGTHRLYSLYSCYSMETGLNLYSCYSASN
3   ARGGLUHISGLYHIS

Samples:

sample_1: H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz