BMRB Entry 27144

Assembly:

Entities:

Entity 1, c-Myc Pu22 22 residues - Formula weight is not available

22 nt of DNA with two compounds

Entity 2, DC34, 1 - C24 H25 F3 N2 O3 - 446.462 Da.

Samples:

sample_1: c-Myc Pu22 0.3 mM; Tris, D11, 25 mM; potassium chloride 50 mM

sample_2: c-Myc Pu22 .125 mM; DC34 .25 mM; Tris, D11, 25 mM; potassium chloride 50 mM

sample_3: c-Myc Pu22 .25 mM; DC34 .5 mM; Tris, D11, 25 mM; potassium chloride 50 mM

sample_4: c-Myc Pu22 .25 mM; DC34, selective 13C-labeling, .5 mM; Tris, D11, 25 mM; potassium chloride 50 mM

sample_5: DC34 0.5 mM; Tris, D11, 25 mM; potassium chloride 50 mM

sample_6: c-Myc Pu22 .1 mM; DC340 – .6 mM; Tris, D11, 25 mM; potassium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 298.2 K

Experiments:

Software:

TOPSPIN v3.5pl5, Bruker Biospin - collection

NMRPipe v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

MestReNova v11.0, Mestrelab Research - data analysis

NMR spectrometers:

• Bruker Avance 850 MHz
• Bruker Avance 800 MHz
• Bruker Avance 700 MHz