BMRB Entry 27358
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27358
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Title: DNA binding protein inhibitor ID2 in 8M urea pH2.3 PubMed: 29642431
Deposition date: 2018-01-15 Original release date: 2018-04-18
Authors: Roschger, Cornelia; Schubert, Mario; Cabrele, Chiara
Citation: Roschger, Cornelia; Schubert, Mario; Regl, Christof; Andosch, Ancuela; Marquez Macheda, Augusto; Berger, Thomas; Huber, Christian; Lutz-Meindl, Ursula; Cabrele, Chiara. "The Recombinant Inhibitor of DNA Binding Id2 Forms Multimeric Structures via the Helix-Loop-Helix Domain and the Nuclear Export Signal" Int. J. Mol. Sci. 19, E1105-E1105 (2018).
Assembly members:
Id2, polymer, 134 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Id2: MKAFSPVRSVRKNSLSDHSL
GISRSKTPVDDPMSLLYNMN
DCYSKLKELVPSIPQNKKVS
KMEILQHVIDYILDLQIALD
SHPTIVSLHHQRPGQNQASR
TPLTTLNTDISILSLQASEF
PSELMSNDSKALCG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 382 |
| 15N chemical shifts | 124 |
| 1H chemical shifts | 129 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Id2 | 1 |
Entities:
Entity 1, Id2 134 residues - Formula weight is not available
| 1 | MET | LYS | ALA | PHE | SER | PRO | VAL | ARG | SER | VAL | ||||
| 2 | ARG | LYS | ASN | SER | LEU | SER | ASP | HIS | SER | LEU | ||||
| 3 | GLY | ILE | SER | ARG | SER | LYS | THR | PRO | VAL | ASP | ||||
| 4 | ASP | PRO | MET | SER | LEU | LEU | TYR | ASN | MET | ASN | ||||
| 5 | ASP | CYS | TYR | SER | LYS | LEU | LYS | GLU | LEU | VAL | ||||
| 6 | PRO | SER | ILE | PRO | GLN | ASN | LYS | LYS | VAL | SER | ||||
| 7 | LYS | MET | GLU | ILE | LEU | GLN | HIS | VAL | ILE | ASP | ||||
| 8 | TYR | ILE | LEU | ASP | LEU | GLN | ILE | ALA | LEU | ASP | ||||
| 9 | SER | HIS | PRO | THR | ILE | VAL | SER | LEU | HIS | HIS | ||||
| 10 | GLN | ARG | PRO | GLY | GLN | ASN | GLN | ALA | SER | ARG | ||||
| 11 | THR | PRO | LEU | THR | THR | LEU | ASN | THR | ASP | ILE | ||||
| 12 | SER | ILE | LEU | SER | LEU | GLN | ALA | SER | GLU | PHE | ||||
| 13 | PRO | SER | GLU | LEU | MET | SER | ASN | ASP | SER | LYS | ||||
| 14 | ALA | LEU | CYS | GLY |
Samples:
sample_1: Id2, [U-99% 13C; U-99% 15N], 0.74 mM; urea 8 M; chloride 180 mM
sample_conditions_1: ionic strength: 180 mM; pH: 2.3; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)NCANH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.2, Bruker Biospin - processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts