BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27433

Title: Double knot toxin (DkTx)

Deposition date: 2018-03-25 Original release date: 2019-03-18

Authors: Ramanujam, Venkatraman; Mobli, Mehdi

Citation: Ramanujam, Venkatraman; Ma, Linlin; King, Glenn; Mobli, Mehdi. "Production and characterization of double knot toxin (DkTx), a TrpV1 channel agonist."  Not known ., .-..

Assembly members:
Double_knot_toxin, polymer, 78 residues, Formula weight is not available

Natural source:   Common Name: Chinese earth tiger   Taxonomy ID: 29017   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ornithoctonus huwena

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Double_knot_toxin: GDCAKEGEVCSWGKKCCDLD NFYCPMEFIPHCKKYKPYVP VTTSFNCAKEGEVCGWGSKC CHGLDCPLAFIPYCEKYR

Data sets:
Data typeCount
13C chemical shifts347
15N chemical shifts75
1H chemical shifts519

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Double knot toxin1

Entities:

Entity 1, Double knot toxin 78 residues - Formula weight is not available

1   GLYASPCYSALALYSGLUGLYGLUVALCYS
2   SERTRPGLYLYSLYSCYSCYSASPLEUASP
3   ASNPHETYRCYSPROMETGLUPHEILEPRO
4   HISCYSLYSLYSTYRLYSPROTYRVALPRO
5   VALTHRTHRSERPHEASNCYSALALYSGLU
6   GLYGLUVALCYSGLYTRPGLYSERLYSCYS
7   CYSHISGLYLEUASPCYSPROLEUALAPHE
8   ILEPROTYRCYSGLULYSTYRARG

Samples:

sample_1: Double knot toxin, [U-13C; U-15N], 300 uM; sodium phosphate 20 mM; DSS 10 uM; sodium azide 0.02 % w/v

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CCPNMR, CCPN - data analysis

NMR spectrometers:

  • Bruker Avance 900 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts