BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 27530

Title: apoCaM bound to Cav1.2 IQ

Deposition date: 2018-06-28 Original release date: 2019-09-17

Authors: Turner, Matthew; Ames, James; Anderson, David

Citation: Turner, Matthew; Ames, James; Anderson, David. "ApoCaM bound to Cav1.2 IQ"  Cell ., .-..

Assembly members:
apoCaM, polymer, 149 residues, Formula weight is not available

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
apoCaM: MADQLTEEQIAEFKEAFSLF DKDGDGTITTKELGTVMRSL GQNPTEAELQDMINEVDADG NGTIDFPEFLTMMARKMKDT DSEEEIREAFRVFDKDGNGY ISAAELRHVMTNLGEKLTDE EVDEMIREADIDGDGQVNYE EFVQMMTAK

Data sets:
Data typeCount
15N chemical shifts115
1H chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apoCaM1

Entities:

Entity 1, apoCaM 149 residues - Formula weight is not available

1   METALAASPGLNLEUTHRGLUGLUGLNILE
2   ALAGLUPHELYSGLUALAPHESERLEUPHE
3   ASPLYSASPGLYASPGLYTHRILETHRTHR
4   LYSGLULEUGLYTHRVALMETARGSERLEU
5   GLYGLNASNPROTHRGLUALAGLULEUGLN
6   ASPMETILEASNGLUVALASPALAASPGLY
7   ASNGLYTHRILEASPPHEPROGLUPHELEU
8   THRMETMETALAARGLYSMETLYSASPTHR
9   ASPSERGLUGLUGLUILEARGGLUALAPHE
10   ARGVALPHEASPLYSASPGLYASNGLYTYR
11   ILESERALAALAGLULEUARGHISVALMET
12   THRASNLEUGLYGLULYSLEUTHRASPGLU
13   GLUVALASPGLUMETILEARGGLUALAASP
14   ILEASPGLYASPGLYGLNVALASNTYRGLU
15   GLUPHEVALGLNMETMETTHRALALYS

Samples:

sample_1: apoCaM, [U-100% 15N], 350 uM; apoCaM, [U-100% 13C; U-100% 15N], 350 uM

sample_conditions_1: ionic strength: 0.150 M; pH: 7.3; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts