BMRB Entry 27683
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27683
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Title: Euprosthenops australis major ampullate spidroin 1 N-terminal domain (NTD) mutant at pH7 PubMed: 31558722
Deposition date: 2018-11-06 Original release date: 2019-09-04
Authors: Goretzki, Benedikt; Heiby, Julia; Hellmich, Ute A.; Neuweiler, Hannes
Citation: Heiby, Julia; Goretzki, Benedikt; Johnson, Christopher; Hellmich, Ute; Neuweiler, Hannes. "Methionine in a protein hydrophobic core drives tight interactions required for assembly of spider silk" Nat. Commun. 10, 4378-4378 (2019).
Assembly members:
MaSp1_L6-NTD, polymer, 137 residues, 14071.6 Da.
Natural source: Common Name: Euprosthenops australis Taxonomy ID: 332052 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Euprosthenops australis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MaSp1_L6-NTD: GSGNSHTTPWTNPGLAENFL
NSFLQGLSSMPGFTASQLDD
LSTIAQSLVQSIQSLAAQGR
TSPNKLQALNMAFASSLAEI
AASEEGGGSLSTKTSSIASA
LSNAFLQTTGVVNQPFINEI
TQLVSMFAQAGMNDVSA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 513 |
| 15N chemical shifts | 126 |
| 1H chemical shifts | 827 |
Additional metadata:
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