BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30032

Title: Solution Structure of Miz-1 Zinc Finger 13   PubMed: 26972249

Deposition date: 2016-03-08 Original release date: 2016-03-28

Authors: Tremblay, C.; Bedard, M.; Lavigne, P.

Citation: Tremblay, C.; Bedard, M.; Bonin, MA.; Lavigne, P.. "Solution structure of the 13th C2H2 Zinc Finger of Miz-1"  Biochem. Biophys. Res. Commun. 473, 471-475 (2016).

Assembly members:
entity_1, polymer, 29 residues, 3232.629 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: HILYACDSCGDKFLDANSLA QHVRIHTAQ

Data sets:
Data typeCount
13C chemical shifts65
15N chemical shifts22
1H chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 29 residues - 3232.629 Da.

1   HISILELEUTYRALACYSASPSERCYSGLY
2   ASPLYSPHELEUASPALAASNSERLEUALA
3   GLNHISVALARGILEHISTHRALAGLN

Entity 2, entity_2 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: ZF 13 0.75 mM; ZnCl2 2 mM; KCl 50 mM; Bis Tris, [U-2H], 10 mM; TECP 2 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

ARIA v2.2, Linge, O'Donoghue and Nilges - structure calculation

CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CcpNMR_Analysis, CCPN - chemical shift assignment

DANGLE v1.1, CCPN - data analysis

NMRPipe v7.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Unity Inova 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts