BMRB Entry 30077
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PDB ID: 5js7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30077
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Title: Structural model of a apo G-protein alpha subunit determined with NMR residual dipolar couplings and SAXS PubMed: 27298341
Deposition date: 2016-05-07 Original release date: 2016-06-24
Authors: Goricanec, D.; Stehle, R.; Grigoriu, S.; Wagner, G.; Hagn, F.
Citation: Goricanec, D.; Stehle, R.; Egloff, P.; Grigori, S.; Plueckthun, A.; Wagner, G.; Hagn, F.. "Conformational dynamics of a G-protein alpha subunit is tightly regulated by nucleotide binding" Proc. Natl. Acad. Sci. U. S. A. 113, E3629-E3638 (2016).
Assembly members:
Guanine nucleotide-binding protein G(i) subunit alpha-1, polymer, 326 residues, 37247.414 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Entity Sequences (FASTA):
Guanine nucleotide-binding protein G(i) subunit alpha-1: GASREVKLLLLGAGESGKST
IVKQMKIIHEAGYSEEECKQ
YKAVVYSNTIQSIIAIIRAM
GRLKIDFGDSARADDARQLF
VLAGAAEEGFMTAELAGVIK
RLWKDSGVQACFNRSREYQL
NDSAAYYLNDLDRIAQPNYI
PTQQDVLRTRVKTTGIVETH
FTFKDLHFKMFDVGGQRSER
KKWIHCFEGVAAIIFCVALS
DYDLVLAEDEEMNRMHESMK
LFDSICNNKWFTDTSIILFL
NKKDLFEEKIKKSPLTICYQ
EYAGSNTYEEAAAYIQCQFE
DLNKRKDTKEIYTHFTCATD
TKNVQFVFDAVTDVIIKNNL
KDCGLF
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 244 |
| 1H chemical shifts | 244 |
Additional metadata:
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