BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 30233

Title: ov-GRN12-34

Deposition date: 2017-01-18 Original release date: 2018-01-17

Authors: Bansal, P.; Smout, M.; Wilson, D.; Caceres, C.; Dastpeyman, M.; Sotillo, J.; Seifert, J.; Brindley, P.; Loukas, A.; Daly, N.

Citation: Bansal, P.; Smout, M.; Wilson, D.; Caceres, C.; Dastpeyman, M.; Sotillo, J.; Seifert, J.; Brindley, P.; Loukas, A.; Daly, N.. "ov-GRN12-34"  . ., .-..

Assembly members:
Granulin, polymer, 23 residues, 2457.811 Da.

Natural source:   Common Name: flatworms   Taxonomy ID: 6198   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Opisthorchis viverrini

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Granulin: CPDPVYTCRPGQTCARGLHG YGC

Data sets:
Data typeCount
13C chemical shifts45
15N chemical shifts22
1H chemical shifts136

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 23 residues - 2457.811 Da.

1   CYSPROASPPROVALTYRTHRCYSARGPRO
2   GLYGLNTHRCYSALAARGGLYLEUHISGLY
3   TYRGLYCYS

Samples:

sample_1: D2O 10%; H2O 90%; ov-GRN12-34 0.5 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1anisotropicsample_conditions_1
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D 1H-1H COSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-15N HSQCsample_1anisotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNMR, CCPN - chemical shift assignment, data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN v3.5PL6, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts