BMRB Entry 30370

Name: Solution NMR structure of uncharacterized protein YejG representing the first structure from PF13989
Published: 2018-10-31

Click here to enlarge.

PDB ID: 6bi6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30370
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Solution NMR structure of uncharacterized protein YejG representing the first structure from PF13989 PubMed: 30958578

Deposition date: 2017-11-01 Original release date: 2018-10-31

Authors: Mohanty, B.; Finn, T.; Macindoe, I.; Zhong, J.; Patrick, W.; Mackay, J.

Citation: Mohanty, Biswaranjan; Hanson-Manful, Paulina; Finn, Thomas; Chambers, Cecilia; McKellar, James; Macindoe, Ingrid; Helder, Stephanie; Setiyaputra, Surya; Zhong, Yichen; Mackay, Joel; Patrick, Wayne. "The uncharacterized bacterial protein YejG has the same architecture as domain III of elongation factor G." Proteins 87, 699-705 (2019).

Assembly members:
entity_1, polymer, 115 residues, 12557.149 Da.

Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GPTSLQLSIVHRLPQNYRWS AGFAGSKVEPIPQNGPCGDN SLVALKLLSPDGDNAWSVMY KLSQALSDIEVPCSVLECEG EPCLFVNRQDEFAATCRLKN FGVAIAEPFSNYNPF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	439
15N chemical shifts	106
1H chemical shifts	689

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 115 residues - 12557.149 Da.

1

GLY

PRO

THR

SER

LEU

GLN

LEU

SER

ILE

VAL

2

HIS

ARG

LEU

PRO

GLN

ASN

TYR

ARG

TRP

SER

3

ALA

GLY

PHE

ALA

GLY

SER

LYS

VAL

GLU

PRO

4

ILE

PRO

GLN

ASN

GLY

PRO

CYS

GLY

ASP

ASN

5

SER

LEU

VAL

ALA

LEU

LYS

LEU

SER

PRO

6

ASP

GLY

ASP

ASN

ALA

TRP

SER

VAL

MET

TYR

7

LYS

LEU

SER

GLN

ALA

LEU

SER

ASP

ILE

GLU

8

VAL

PRO

CYS

SER

VAL

LEU

GLU

CYS

GLU

GLY

9

GLU

PRO

CYS

LEU

PHE

VAL

ASN

ARG

GLN

ASP

10

GLU

PHE

ALA

THR

CYS

ARG

LEU

LYS

ASN

11

PHE

GLY

VAL

ALA

ILE

ALA

GLU

PRO

PHE

SER

12

ASN

TYR

ASN

PRO

PHE

Samples:

sample_1: Bi-tris 20 mM; NaCl 50 mM; PMSF 0.2 mM; TCEP 1 mM; YejG, [U-13C; U-15N], 600 uM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D [15N,1H]-HSQC	sample_1	not available	sample_conditions_1
3D 15N-resolved [1H,1H]-NOESY	sample_1	not available	sample_conditions_1
3D 13Cali-resolved [1H,1H]-NOESY	sample_1	not available	sample_conditions_1
3D 13Caro-resolved [1H,1H]-NOESY	sample_1	not available	sample_conditions_1
2D [13Cali,1H]-HSQC (multiplicity edited)	sample_1	not available	sample_conditions_1
2D [13Caro,1H]-HSQC	sample_1	not available	sample_conditions_1
2D 1H-1H NOESY	sample_1	not available	sample_conditions_1
3D NUS HNCA	sample_1	not available	sample_conditions_1
3D NUS HNCACB	sample_1	not available	sample_conditions_1
3D NUS CBCA(CO)NH	sample_1	not available	sample_conditions_1
3D NUS HN(CA)CO	sample_1	not available	sample_conditions_1
3D NUS HNCO	sample_1	not available	sample_conditions_1
3D NUS HBHA(CO)NH	sample_1	not available	sample_conditions_1
3D NUS HNHA	sample_1	not available	sample_conditions_1
3D NUS (H)CC(CO)NH	sample_1	not available	sample_conditions_1
15N{1H}-NOE	sample_1	not available	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

OPALp, Koradi, Billeter and Guntert - refinement

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance III 600 MHz
Bruker Avance III 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts