BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30410

Title: Solution structure of the four-helix bundle region of human J-protein Zuotin, a component of ribosome-associated complex (RAC)   PubMed: 31091298

Deposition date: 2018-02-20 Original release date: 2019-06-07

Authors: Shrestha, O.; Lee, W.; Tonelli, M.; Cornilescu, G.; Markley, J.; Ciesielski, S.; Craig, E.

Citation: Shrestha, Om Kumar; Sharma, Ruchika; Tomiczek, Bartlomiej; Lee, Woonghee; Tonelli, Marco; Cornilescu, Gabriel; Stolarska, Milena; Nierzwicki, Lukasz; Czub, Jacek; Markley, John; Marszalek, Jaroslaw; Ciesielski, Szymon; Craig, Elizabeth. "Structure and Evolution of the 4-helix Bundle Domain of Zuotin, a J-domain Protein Co-Chaperone of Hsp70"  PLoS ONE 14, e0217098-e0217098 (2019).

Assembly members:
entity_1, polymer, 89 residues, 10401.884 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GSKKAIKKERQKLRNSCKTW NHFSDNEAERVKMMEEVEKL CDRLELASLQCLNETLTSCT KEVGKAALEKQIEEINEQIR KEKEEAEAR

Data sets:
Data typeCount
13C chemical shifts268
15N chemical shifts85
1H chemical shifts521

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 89 residues - 10401.884 Da.

1   GLYSERLYSLYSALAILELYSLYSGLUARG
2   GLNLYSLEUARGASNSERCYSLYSTHRTRP
3   ASNHISPHESERASPASNGLUALAGLUARG
4   VALLYSMETMETGLUGLUVALGLULYSLEU
5   CYSASPARGLEUGLULEUALASERLEUGLN
6   CYSLEUASNGLUTHRLEUTHRSERCYSTHR
7   LYSGLUVALGLYLYSALAALALEUGLULYS
8   GLNILEGLUGLUILEASNGLUGLNILEARG
9   LYSGLULYSGLUGLUALAGLUALAARG

Samples:

sample_1: Mpp11 (346-432), [U-100% 13C; U-100% 15N], 300 uM; NaCl 250 mM; sodium phosphate 20 mM

sample_2: Mpp11 (346-432), [U-100% 15N], 300 uM; NaCl 250 mM; sodium phosphate 20 mM

sample_3: Mpp11 (346-432), [U-100% 13C; U-100% 15N], 200 uM; NaCl 250 mM; sodium phosphate 20 mM

sample_4: Mpp11 (346-432), [U-100% 13C; U-100% 15N], 150 uM; NaCl 250 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 270 mM; pH: 7.5; pressure: 1 Pa; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
1H-15N IPAP HSQCsample_1isotropicsample_conditions_1
1H-15N IPAP HSQCsample_3anisotropicsample_conditions_1
1H-15N IPAP HSQCsample_4anisotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

VNMR, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

APES, Shin, Lee and Lee - peak picking

NMRFAM-SPARKY, Lee, Tonelli and Markley - peak picking

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

PINE-SPARKY, Lee, Westler, Bahrami, Eghbalnia and Markley - chemical shift assignment

PONDEROSA-C/S, Lee, Stark and Markley - refinement, structure calculation

AUDANA, Lee, Petit, Cornilescu, Stark and Markley - structure calculation

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

  • Varian VNS 600 MHz
  • Bruker Avance 750 MHz
  • Bruker Avance 600 MHz
  • Varian VNS 900 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts